Response of mice and ferrets to a monovalent influenza A (H7N9) split vaccine

In early spring 2013, the emergence of the influenza A (H7N9) virus in humans in Eastern China raised concerns of a new influenza pandemic. Development of a safe and effective H7N9 influenza vaccine is urgently needed. To this end, we first synthesized the hemagglutinin (HA) and neuraminidase (NA) g...

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Bibliographic Details
Published in:PloS one 2014-06, Vol.9 (6), p.e99322-e99322
Main Authors: Duan, Yueqiang, Gu, Hongjing, Chen, Rui, Zhao, Zhongpeng, Zhang, Liangyan, Xing, Li, Lai, Chengcai, Zhang, Peirui, Li, Zhiwei, Zhang, Keming, Wang, Zhouhai, Zhang, Shaogeng, Wang, Xiliang, Yang, Penghui
Format: Article
Language:English
Subjects:
Animals
Capsid - ultrastructure
Cercopithecus aethiops
Chickens
Eggs
Epidemiology
Exo-a-sialidase
Female
Ferrets
Genes
Genetics
Hemagglutinins
Humans
Immunogenicity
Influenza
Influenza A
Influenza A Virus, H7N9 Subtype - genetics
Influenza A Virus, H7N9 Subtype - immunology
Influenza A Virus, H7N9 Subtype - ultrastructure
Influenza Vaccines - immunology
Influenza, Human - immunology
Influenza, Human - prevention & control
Influenza, Human - virology
Laboratories
Medicine and Health Sciences
Mice
Mice, Inbred BALB C
Pandemics
Pathogenicity
Pathogens
Plasmids
Proteins
Reverse Genetics
Vaccination
Vaccines
Vaccines, Attenuated - immunology
Vero Cells
Virus Replication
Viruses
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Summary:In early spring 2013, the emergence of the influenza A (H7N9) virus in humans in Eastern China raised concerns of a new influenza pandemic. Development of a safe and effective H7N9 influenza vaccine is urgently needed. To this end, we first synthesized the hemagglutinin (HA) and neuraminidase (NA) genes of the influenza A (H7N9) virus A/AnHui/1/2013. Using reverse genetics, we rescued a reassortant virus (H7N9/PR8) that contained the HA and NA genes from wild-type H7N9 and six genes encoding internal proteins from the A/Puerto Rico/8/34 (PR8) virus. Next, the pathogenicity of the reassortant virus was evaluated both in vivo and in vitro. We found that the virus was non-pathogenic in mice and was stable after serial passaging in eggs. Furthermore, we found that a monovalent influenza A (H7N9) split vaccine prepared from the virus was immunogenic in mice and ferrets. When given intramuscularly, the vaccine (two doses of at least 15-µg) completely protected mice from normally lethal wild-type H7N9 virus challenge. In summary, our H7N9 vaccine, developed over a short time, is a potential candidate for further clinical evaluation and human use.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0099322