A high throughput biochemical fluorometric method for measuring lipid peroxidation in HDL

Current cell-based assays for determining the functional properties of high-density lipoproteins (HDL) have limitations. We report here the development of a new, robust fluorometric cell-free biochemical assay that measures HDL lipid peroxidation (HDLox) based on the oxidation of the fluorochrome Am...

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Bibliographic Details
Published in:PloS one 2014-11, Vol.9 (11), p.e111716-e111716
Main Authors: Kelesidis, Theodoros, Roberts, Christian K, Huynh, Diana, Martínez-Maza, Otoniel, Currier, Judith S, Reddy, Srinivasa T, Yang, Otto O
Format: Article
Language:English
Subjects:
Adolescent
Adult
Animal models
Animals
Anthropometry
Apolipoproteins
Arteriosclerosis
Assaying
Atherosclerosis
Biology and Life Sciences
Cardiovascular diseases
Carotid Intima-Media Thickness
Cholesterol
Coronary Artery Disease - blood
Coronary Artery Disease - metabolism
Correlation analysis
Female
Fluorometry - methods
Gynecology
Heart diseases
High density lipoprotein
High-Throughput Screening Assays - methods
HIV
HIV Infections - blood
HIV Infections - metabolism
HIV-1 - isolation & purification
Human immunodeficiency virus
Humans
Immunology
Laboratories
Lipid Peroxidation
Lipids
Lipoproteins
Lipoproteins (high density)
Lipoproteins, HDL - blood
Lipoproteins, HDL - chemistry
Lipoproteins, HDL - metabolism
Low density lipoprotein
Male
Medical research
Medicine
Medicine and Health Sciences
Metabolism
Mice, Inbred C57BL
Middle Aged
Obstetrics
Oxazines - analysis
Oxazines - metabolism
Oxidation
Peroxidation
Studies
Viability
Viruses
Young Adult
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Summary:Current cell-based assays for determining the functional properties of high-density lipoproteins (HDL) have limitations. We report here the development of a new, robust fluorometric cell-free biochemical assay that measures HDL lipid peroxidation (HDLox) based on the oxidation of the fluorochrome Amplex Red. HDLox correlated with previously validated cell-based (r = 0.47, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0111716