Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower

Sclerotinia Head Rot (SHR), a disease caused by Sclerotinia sclerotiorum, is one of the most limiting factors in sunflower production. In this study, we identified genomic loci associated with resistance to SHR to support the development of assisted breeding strategies. We genotyped 114 Recombinant...

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Bibliographic Details
Published in:PloS one 2017-12, Vol.12 (12), p.e0189859-e0189859
Main Authors: Zubrzycki, Jeremías Enrique, Maringolo, Carla Andrea, Filippi, Carla Valeria, Quiróz, Facundo José, Nishinakamasu, Verónica, Puebla, Andrea Fabiana, Di Rienzo, Julio A, Escande, Alberto, Lia, Verónica Viviana, Heinz, Ruth Amalia, Hopp, Horacio Esteban, Cervigni, Gerardo D L, Paniego, Norma Beatriz
Format: Article
Language:English
Subjects:
Analysis
Ascomycota - physiology
Ascospores
Biology and Life Sciences
Breeding
Chromosome Mapping
Disease
Disease control
Disease resistance (Plants)
Disease Resistance - genetics
Epistasis
Epistasis, Genetic
Gene loci
Gene mapping
Gene polymorphism
Genetic aspects
Genetic Linkage
Genetic Markers
Genotype
Head rot
Helianthus
Helianthus - genetics
Helianthus - microbiology
Helianthus annuus
Heritability
Inbreeding
Incubation
Limiting factors
Loci
Mapping
Medicine and Health Sciences
Phenotype
Physiological aspects
Plant Diseases - microbiology
Polymorphism
Polymorphism, Single Nucleotide - genetics
Quantitative trait loci
Quantitative Trait Loci - genetics
Research and Analysis Methods
Sclerotinia sclerotiorum
Single nucleotide polymorphisms
Single-nucleotide polymorphism
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Summary:Sclerotinia Head Rot (SHR), a disease caused by Sclerotinia sclerotiorum, is one of the most limiting factors in sunflower production. In this study, we identified genomic loci associated with resistance to SHR to support the development of assisted breeding strategies. We genotyped 114 Recombinant Inbred Lines (RILs) along with their parental lines (PAC2 -partially resistant-and RHA266 -susceptible-) by using a 384 single nucleotide polymorphism (SNP) Illumina Oligo Pool Assay to saturate a sunflower genetic map. Subsequently, we tested these lines for SHR resistance using assisted inoculations with S. sclerotiorum ascospores. We also conducted a randomized complete-block assays with three replicates to visually score disease incidence (DI), disease severity (DS), disease intensity (DInt) and incubation period (IP) through four field trials (2010-2014). We finally assessed main effect quantitative trait loci (M-QTLs) and epistatic QTLs (E-QTLs) by composite interval mapping (CIM) and mixed-model-based composite interval mapping (MCIM), respectively. As a result of this study, the improved map incorporates 61 new SNPs over candidate genes. We detected a broad range of narrow sense heritability (h2) values (1.86-59.9%) as well as 36 M-QTLs and 13 E-QTLs along 14 linkage groups (LGs). On LG1, LG10, and LG15, we repeatedly detected QTLs across field trials; which emphasizes their putative effectiveness against SHR. In all selected variables, most of the identified QTLs showed high determination coefficients, associated with moderate to high heritability values. Using markers shared with previous Sclerotinia resistance studies, we compared the QTL locations in LG1, LG2, LG8, LG10, LG11, LG15 and LG16. This study constitutes the largest report of QTLs for SHR resistance in sunflower. Further studies focusing on the regions in LG1, LG10, and LG15 harboring the detected QTLs are necessary to identify causal alleles and contribute to unraveling the complex genetic basis governing the resistance.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0189859