Changes in the interstitial cells of Cajal and neuronal nitric oxide synthase positive neuronal cells with aging in the esophagus of F344 rats

The aging-associated cellular and molecular changes in esophagus have not been established, yet. Thus we evaluated histological structure, interstitial cells of Cajal (ICCs), neuronal nitric oxide synthase (nNOS)-positive cells, and contractility in the esophagus of Fischer 344 rat at different ages...

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Published in:PloS one 2017-11, Vol.12 (11), p.e0186322-e0186322
Main Authors: Kim, Hee Jin, Kim, Nayoung, Kim, Yong Sung, Nam, Ryoung Hee, Lee, Sun Min, Park, Ji Hyun, Choi, Daeun, Hwang, Young-Jae, Lee, Jongchan, Lee, Hye Seung, Kim, Min-Seob, Lee, Moon Young, Lee, Dong Ho
Format: Article
Language:English
Subjects:
Age
Aging
Aging (Biology)
Biology and Life Sciences
c-Kit protein
Enteric nervous system
Esophageal diseases
Esophagus
Experiments
Force measurement
Gastroenterology
Gene expression
Hospitals
Immunohistochemistry
Internal medicine
Interstitial cells
Interstitial cells of Cajal
KIT protein
Lamina propria
Leydig cells
Medicine
Medicine and Health Sciences
Motility
Muscle contraction
Muscles
Neurons
Nitric oxide
Nitric-oxide synthase
Pathogens
Physiological aspects
Physiology
Rats
Research and Analysis Methods
Rodents
Smooth muscle
Stem cell factor
Stem cells
Stomach
Studies
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Summary:The aging-associated cellular and molecular changes in esophagus have not been established, yet. Thus we evaluated histological structure, interstitial cells of Cajal (ICCs), neuronal nitric oxide synthase (nNOS)-positive cells, and contractility in the esophagus of Fischer 344 rat at different ages (6-, 31-, 74-weeks, and 2-years). The lamina propria thickness and endomysial area were calculated. The immunoreactivity of c-Kit, nNOS and protein gene product (PGP) 9.5 was counted after immunohistochemistry. Expression of c-Kit, stem cell factor (SCF), nNOS and PGP 9.5 mRNA was measured by real-time PCR, and expression of c-Kit and nNOS protein was detected by Western blot. Isovolumetric contractile force measurement and electrical field stimulation (EFS) were conducted. The lamina propria thickness increased (6 week vs 2 year, P = 0.005) and the endomysial area of longitudinal muscle decreased with aging (6 week vs 2 year, P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0186322