A universal mammalian vaccine cell line substrate

Using genome-wide small interfering RNA (siRNA) screens for poliovirus, influenza A virus and rotavirus, we validated the top 6 gene hits PV, RV or IAV to search for host genes that when knocked-down (KD) enhanced virus permissiveness and replication over wild type Vero cells or HEp-2 cells. The enh...

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Bibliographic Details
Published in:PloS one 2017-11, Vol.12 (11), p.e0188333-e0188333
Main Authors: Murray, Jackelyn, Todd, Kyle V, Bakre, Abhijeet, Orr-Burks, Nichole, Jones, Les, Wu, Weilin, Tripp, Ralph A
Format: Article
Language:English
Subjects:
Animals
Biology and Life Sciences
Cell Line
Cell lines
Chlorocebus aethiops
Diagnostic systems
Gene Knockdown Techniques
Genes
Genes, Viral
Genetic screening
Genome
Genomes
Growth
Immunization
Influenza A
Mammalian cells
Mammals
Mammals - metabolism
Medicine and health sciences
Physiological aspects
Production processes
Real-Time Polymerase Chain Reaction
Replication
Research and Analysis Methods
Ribonucleic acid
RNA
RNA interference
RNA, Small Interfering - metabolism
Rotavirus
siRNA
Substrates
Vaccines
Vaccines - metabolism
Vero Cells
Veterinary colleges
Virus Replication - genetics
Viruses
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Summary:Using genome-wide small interfering RNA (siRNA) screens for poliovirus, influenza A virus and rotavirus, we validated the top 6 gene hits PV, RV or IAV to search for host genes that when knocked-down (KD) enhanced virus permissiveness and replication over wild type Vero cells or HEp-2 cells. The enhanced virus replication was tested for 12 viruses and ranged from 2-fold to >1000-fold. There were variations in virus-specific replication (strain differences) across the cell lines examined. Some host genes (CNTD2, COQ9, GCGR, NDUFA9, NEU2, PYCR1, SEC16G, SVOPL, ZFYVE9, and ZNF205) showed that KD resulted in enhanced virus replication. These findings advance platform-enabling vaccine technology, the creation of diagnostic cells substrates, and are informative about the host mechanisms that affect virus replication in mammalian cells.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0188333