Restitution characteristics of His bundle and working myocardium in isolated rabbit hearts

The Purkinje system (PS) and the His bundle have been recently implicated as an important driver of the rapid activation rate after 1-2 minutes of ventricular fibrillation (VF). It is unknown whether activations during VF propagate through the His-Purkinje system to other portions of the the working...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2017-10, Vol.12 (10), p.e0186880-e0186880
Main Authors: Huang, Shangwei, Wu, Liqun, Huang, Jian, Panitchob, Nuttanont, Hu, Nan, Ranjan, Ravi, Dosdall, Derek J
Format: Article
Language:English
Subjects:
Action Potentials
Animals
Biology and Life Sciences
Bundle of His - physiology
Bundling
Cardiac arrhythmia
Cardiology
Electrodes
Engineering and Technology
Fibrillation
Genetic aspects
Heart
His bundle
In Vitro Techniques
Internal medicine
Laboratory animals
Medicine
Medicine and Health Sciences
Microelectrodes
Myocardium
Myocardium - metabolism
Papillary muscles
Physical Sciences
Physiological aspects
Physiology
Rabbits
Research and Analysis Methods
Restitution
Rodents
Salt
Ventricle
Ventricular fibrillation
Wave fronts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Purkinje system (PS) and the His bundle have been recently implicated as an important driver of the rapid activation rate after 1-2 minutes of ventricular fibrillation (VF). It is unknown whether activations during VF propagate through the His-Purkinje system to other portions of the the working myocardium (WM). Little is known about restitution characteristic differences between the His bundle and working myocardium at short cycle lengths. In this study, rabbit hearts (n = 9) were isolated, Langendorff-perfused, and electromechanically uncoupled with blebbistatin (10 μM). Pacing pulses were delivered directly to the His bundle. By using standard glass microelectrodes, action potentials duration (APD) from the His bundle and WM were obtained simultaneously over a wide range of stimulation cycle lengths (CL). The global F-test indicated that the two restitution curves of the His bundle and the WM are statistically significantly different (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0186880