Electrochemically generated acid and its containment to 100 micron reaction areas for the production of DNA microarrays

An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron dia...

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Bibliographic Details
Published in:PloS one 2006-12, Vol.1 (1), p.e34-e34
Main Authors: Maurer, Karl, Cooper, John, Caraballo, Marcelo, Crye, James, Suciu, Dominic, Ghindilis, Andrey, Leonetti, Joseph A, Wang, Wei, Rossi, Francis M, Stöver, Axel G, Larson, Christopher, Gao, Hetian, Dill, Kilian, McShea, Andy
Format: Article
Language:English
Subjects:
Acids
Base Sequence
Biochemistry
Biotechnology
Biotechnology/Small Molecule Chemistry
Chemistry/Organic Chemistry
Containment
Deoxyribonucleic acid
DNA
DNA biosynthesis
DNA microarrays
DNA Primers - genetics
Electrochemical Techniques - methods
Electrochemistry
Electrodes
Genetics and Genomics
Indicators and Reagents
Microchemistry
Microelectrodes
Oligodeoxyribonucleotides - chemical synthesis
Oligodeoxyribonucleotides - chemistry
Oligonucleotide Array Sequence Analysis - instrumentation
Oligonucleotide Array Sequence Analysis - methods
Overlaying
Semiconductors
Synthesis
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Summary:An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron diameter was achieved by the presence of an organic base. This procedure was then used for the production of a DNA array, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. The product array was found to have a detection sensitivity to as low as 0.5 pM DNA in a complex background sample.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0000034