Identification of novel antibody-reactive detection sites for comprehensive gluten monitoring

Certain cereals like wheat, rye or barley contain gluten, a protein mixture that can trigger celiac disease (CD). To make gluten-free diets available for affected individuals the gluten content of foodstuff must be monitored. For this purpose, antibody-based assays exist which rely on the recognitio...

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Bibliographic Details
Published in:PloS one 2017-07, Vol.12 (7), p.e0181566-e0181566
Main Authors: Röckendorf, Niels, Meckelein, Barbara, Scherf, Katharina A, Schalk, Kathrin, Koehler, Peter, Frey, Andreas
Format: Article
Language:English
Subjects:
Alcohol
Amino Acid Motifs
Animals
Antibodies - chemistry
Antibodies, Monoclonal - chemistry
Asthma
Autoimmune diseases
B-Lymphocytes - cytology
Barley
Binding sites
Binding sites (Biochemistry)
Biology and Life Sciences
Cadmium
Celiac disease
Celiac Disease - immunology
Cereals
Diagnostic systems
Diet
Diet, Gluten-Free
Edible Grain - chemistry
Epitopes - chemistry
Female
Fermentation
Fermented food
Food
Food processing
Fragments
Gliadin - chemistry
Gluten
Glutens - chemistry
Glutens - immunology
Immunization
Immunoglobulin G - chemistry
Immunology
Medicine and Health Sciences
Methods
Mice
Monoclonal antibodies
Oligonucleotide Array Sequence Analysis
Patients
Peptides
Physiological aspects
Properties
Proteins
Proteomics
Quantitation
Reference materials
Research and Analysis Methods
Rye
Target recognition
Triticum - chemistry
Wheat
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Summary:Certain cereals like wheat, rye or barley contain gluten, a protein mixture that can trigger celiac disease (CD). To make gluten-free diets available for affected individuals the gluten content of foodstuff must be monitored. For this purpose, antibody-based assays exist which rely on the recognition of certain linear gluten sequence motifs. Yet, not all CD-active gluten constituents and fragments formed during food processing/fermentation may be covered by those tests. In this study, we therefore assayed the coverage of reportedly CD-active gluten components by currently available detection antibodies and determined the antibody-inducing capacity of wheat gluten constituents in order to provide novel diagnostic targets for comprehensive gluten quantitation. Immunizations of outbred mice with purified gliadins and glutenins were conducted and the linear target recognition profile of the sera was recorded using synthetic peptide arrays that covered the sequence space of gluten constituents present in those preparations. The resulting murine immunorecognition profile of gluten demonstrated that further linear binding sites beyond those recognized by the monoclonal antibodies α20, R5 and G12 exist and may be exploitable as diagnostic targets. We conclude that the safety of foodstuffs for CD patients can be further improved by complementing current tests with antibodies directed against additional CD-active gluten components. Currently unrepresented linear gluten detection sites in glutenins and α-gliadins suggest sequences QQQYPS, PQQSFP, QPGQGQQG and QQPPFS as novel targets for antibody generation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0181566