Contribution of molecular analysis to the typification of the non-functioning pituitary adenomas

The WHO Classification of Tumours of Endocrine Organs considers the inmunohistochemical characterization of pituitary adenomas (PA) as mandatory for patient diagnosis. Recent advances in the knowledge of the molecular patterns of these tumours could complement this classification with gene expressio...

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Bibliographic Details
Published in:PloS one 2017-07, Vol.12 (7), p.e0180039-e0180039
Main Authors: Sanchez-Tejada, Laura, Sanchez-Ortiga, Ruth, Lamas, Cristina, Camara, Rosa, Riesgo, Pedro, Fajardo, Carmen, Aranda, Francisco Ignacio, Pico, Antonio
Format: Article
Language:English
Subjects:
Addition polymerization
Argipressin
Argipressin receptors
Autopsies
Biology and Life Sciences
Classification
Corticotropin-releasing hormone
Diagnosis
Dopamine
Dopamine receptors
Endocrinology
Female
Gene expression
Gene Expression Regulation, Neoplastic
Genes
Glands
Hormones
Hormones - genetics
Hormones - metabolism
Hospitals
Humans
Hyperthyroidism
Immunohistochemistry
Male
Medicine and Health Sciences
Middle Aged
NMR
Nuclear magnetic resonance
Organs
Pituitary
Pituitary hormones
Pituitary Neoplasms - classification
Pituitary Neoplasms - diagnosis
Pituitary Neoplasms - genetics
Pituitary Neoplasms - pathology
Polymerase chain reaction
Prevalence
Real time
Receptors
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Releasing
Reproducibility of Results
Research and Analysis Methods
Research projects
Thyroid gland
Tumors
Types
Vasopressin
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Summary:The WHO Classification of Tumours of Endocrine Organs considers the inmunohistochemical characterization of pituitary adenomas (PA) as mandatory for patient diagnosis. Recent advances in the knowledge of the molecular patterns of these tumours could complement this classification with gene expression profiling. Within the context of the Spanish Molecular Registry of Pituitary Adenomas (REMAH), a multicentre clinical-basic research project, we analysed the molecular phenotype of 142 PAs with complete IHC and clinical information. Gene expression levels of all pituitary hormones, type 1 corticotrophin-releasing hormone receptor, dopamine receptors and arginine vasopressin receptor 1b were measured by quantitative real-time polymerase chain reaction. In addition, we used three housekeeping genes for normalization and a pool of nine healthy pituitary glands from autopsies as calibration reference standard. Based on the clinically functioning PA (FPA: somatotroph, corticotroph, thyrotroph and lactotroph adenomas), we established the interquartile range of relative expression for all genes studied in each PA subtype. That allowed molecularly the different PA subtypes, including the clinically non-functioning PA (NFPA). Afterwards, we estimated the concordance of the molecular and immunohistochemical classification with clinical diagnosis in FPA and between them in NFPA. The kappa values were higher in molecular than in immunohistochemical classification in FPA and showed a bad concordance in all NFPA subtypes. According to these results, the molecular characterization of the PA complements the IHC analysis, allowing a better typification of the NFPA.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0180039