The flame retardant DE-71 (a mixture of polybrominated diphenyl ethers) inhibits human differentiated thyroid cell function in vitro

Normal thyroid function is essential for general growth and metabolism, but can be affected by endocrine disrupting chemicals (EDCs). Polybrominated diphenyl ethers (PBDEs) have been used worldwide to reduce flammability in different materials and are suspected to be EDCs. The production of the comm...

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Published in:PloS one 2017-06, Vol.12 (6), p.e0179858-e0179858
Main Authors: Kronborg, Thit Mynster, Hansen, Juliana Frohnert, Rasmussen, Åse Krogh, Vorkamp, Katrin, Nielsen, Claus Henrik, Frederiksen, Marie, Hofman-Bang, Jacob, Hahn, Christoffer Holst, Ramhøj, Louise, Feldt-Rasmussen, Ulla
Format: Article
Language:English
Subjects:
Adenoma - surgery
Adenosine monophosphate
Bioaccumulation
Biology and Life Sciences
Biotechnology
Breastfeeding & lactation
Cell culture
Cells, Cultured
Chemical industry
Chemicals
Coding
Complications and side effects
Cyclic AMP
Cyclic AMP - metabolism
Cytotoxicity
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Endocrine disruptors
Endocrine Disruptors - pharmacology
Endocrinology
Enzyme-Linked Immunosorbent Assay
Ethers
Fire retardant chemicals
Fire retardants
Flame retardants
Flame Retardants - pharmacology
Flammability
Gene expression
Genetic aspects
Halogenated Diphenyl Ethers - pharmacology
Homeostasis
Hormones
Humans
In vitro methods and tests
Inhibition
Medicine and Health Sciences
Metabolism
Monolayers
mRNA
PCB
Phase (cyclic)
Physiological aspects
Polybrominated diphenyl ethers
Polychlorinated biphenyls
Polymerase chain reaction
Radioimmunoassay
Real-Time Polymerase Chain Reaction
Research and Analysis Methods
RNA, Messenger - metabolism
Studies
Thyrocytes
Thyroglobulin
Thyroglobulin - metabolism
Thyroid
Thyroid gland
Thyroid Gland - drug effects
Thyroid Gland - metabolism
Thyroid Neoplasms - surgery
Thyroid-stimulating hormone
Thyroid-stimulating hormone receptors
Thyroidectomy
Toxicity
Toxicology
Two phase
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Summary:Normal thyroid function is essential for general growth and metabolism, but can be affected by endocrine disrupting chemicals (EDCs). Polybrominated diphenyl ethers (PBDEs) have been used worldwide to reduce flammability in different materials and are suspected to be EDCs. The production of the commercial Penta- and OctaBDE mixtures is banned, but DecaBDEs and existing products may leak PBDEs into the environment. Our aim was to investigate the effect of the PentaBDE mixture DE-71 on human thyroid cells in vitro. Primary human thyroid cells were obtained as paraadenomatous tissue and cultured in monolayers. The influence of DE-71 on cyclic adenosine monophosphate (cAMP) and thyroglobulin (Tg) production was examined in the culture medium by competitive radioimmunoassay and enzyme-linked immunosorbent assay, respectively. Real-time quantitative PCR analysis of thyroid-specific genes was performed on the exposed cell cultures. PBDE concentrations were determined in cellular and supernatant fractions of the cultures. DE-71 inhibited Tg-release from TSH-stimulated thyrocytes. At 50 mg/L DE-71, mean Tg production was reduced by 71.9% (range: 8.5-98.7%), and cAMP by 95.1% (range: 91.5-98.8%) compared to controls). Expression of mRNA encoding Tg, TPO and TSHr were significantly inhibited (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0179858