Multi-level characterization of balanced inhibitory-excitatory cortical neuron network derived from human pluripotent stem cells

Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory-inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GA...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2017-06, Vol.12 (6), p.e0178533-e0178533
Main Authors: Nadadhur, Aishwarya G, Emperador Melero, Javier, Meijer, Marieke, Schut, Desiree, Jacobs, Gerbren, Li, Ka Wan, Hjorth, J J Johannes, Meredith, Rhiannon M, Toonen, Ruud F, Van Kesteren, Ronald E, Smit, August B, Verhage, Matthijs, Heine, Vivi M
Format: Article
Language:English
Subjects:
Astrocyte Coculture
Astrocytes
Astrocytes - cytology
Astrocytes - physiology
Biology and Life Sciences
Brain
Brain research
Calcium
Calcium imaging
Cell culture
Cell Differentiation - physiology
Cells, Cultured
Coculture Techniques
Computer and Information Sciences
Confocal microscopy
Density
Differentiation
Disease
Disorders
Electrophysiological Phenomena
Fibroblasts
GABA
GABAergic Neurons - cytology
GABAergic Neurons - physiology
Ganglionic Eminence
Genomics
Glutamate
Glutamatergic transmission
Growth factors
Humans
Imaging
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - physiology
Interneurons
Maturation
Messenger RNA
Microscopy
mRNA
Neural networks
Neurobiology
Neurodevelopmental disorders
Neuroimaging
Neurons
Neurosciences
Pediatrics
Pluripotency
Protocol (computers)
Robustness
Single-Cell Analysis
Specification
Stem cells
γ-Aminobutyric acid
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory-inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GABAergic and glutamatergic cortical neurons. We used two different co-culture models with astrocytes. We show that these cultures have balanced excitatory-inhibitory synaptic identities using confocal microscopy, electrophysiological recordings, calcium imaging and mRNA analysis. These simple and robust protocols offer the opportunity for single-cell to multi-level analysis of patient hiPSC-derived cortical excitatory-inhibitory networks; thereby creating advanced tools to study disease mechanisms underlying neurodevelopmental disorders.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0178533