Yellow-Leaf 1 encodes a magnesium-protoporphyrin IX monomethyl ester cyclase, involved in chlorophyll biosynthesis in rice (Oryza sativa L.)

Magnesium-protoporphyrin IX monomethyl ester cyclase (MPEC) catalyzes the conversion of MPME to divinyl protochlorophyllide (DVpchlide). This is an essential enzyme during chlorophyll (Chl) biosynthesis but details of its function in rice are still lacking. Here, we identified a novel rice mutant ye...

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Bibliographic Details
Published in:PloS one 2017-05, Vol.12 (5), p.e0177989-e0177989
Main Authors: Sheng, Zhonghua, Lv, Yusong, Li, Wei, Luo, Rongjian, Wei, Xiangjin, Xie, Lihong, Jiao, Guiai, Shao, Gaoneng, Wang, Jianlong, Tang, Shaoqing, Hu, Peisong
Format: Article
Language:English
Subjects:
Accumulation
Agriculture
Algae
Analysis
Assembly
Bacteria
Barley
Biology
Biology and Life Sciences
Biosynthesis
Breeding
Catalysis
Chemical synthesis
Chlorophyll
Chlorophyll - biosynthesis
Chloroplasts
Circadian rhythms
Cloning
Color
Conversion
Copper
Crop yield
Crops
Deoxyribonucleic acid
Dimensional analysis
DNA
Enzymes
Enzymes - genetics
Enzymes - metabolism
Evolution
Fluorescence
Fluorescence microscopy
Functional analysis
Gene expression
Genes, Plant
Genetic aspects
Genomes
Homology
Hypoxia
Ions
Kinases
Laboratories
Leaves
Mathematical analysis
Membrane proteins
Microscopy, Electron, Transmission
Mutation
Nitrogen
Organelles
Oryza - genetics
Oryza - growth & development
Oryza - metabolism
Oryza sativa
Oxidation
pH effects
Photosynthesis
Physical Sciences
Physiological aspects
Plant breeding
Plants
Plastids
Proteins
Protoporphyrins - metabolism
Regulators
Research and Analysis Methods
Rice
Senescence
Temperature effects
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Summary:Magnesium-protoporphyrin IX monomethyl ester cyclase (MPEC) catalyzes the conversion of MPME to divinyl protochlorophyllide (DVpchlide). This is an essential enzyme during chlorophyll (Chl) biosynthesis but details of its function in rice are still lacking. Here, we identified a novel rice mutant yellow-leaf 1 (yl-1), which showed decreased Chl accumulation, abnormal chloroplast ultrastructure and attenuated photosynthetic activity. Map-based cloning and over-expression analysis suggested that YL-1 encodes a subunit of MPEC. The YL-1 protein localizes in chloroplasts, and it is mainly expressed in green tissues, with greatest abundance in leaves and young panicles. Results of qRT-PCR showed that Chl biosynthesis upstream genes were highly expressed in the yl-1 mutant, while downstream genes were compromised, indicating that YL-1 plays a pivotal role in the Chl biosynthesis. Furthermore, the expression levels of photosynthesis and chloroplast development genes were also affected. RNA-seq results futher proved that numerous membrane-associated genes, including many plastid membrane-associated genes, have altered expression pattern in the yl-1 mutant, implying that YL-1 is required for plastid membrane stability. Thus, our study confirms a putative MPME cyclase as a novel key enzyme essential for Chl biosynthesis and chloroplast membrane stability in rice.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0177989