SUMO-4: A novel functional candidate in the human placental protein SUMOylation machinery

Small ubiquitin-like modifiers (SUMOs) conjugate to proteins post-translationally, thereby affecting target localization, activity and stability. Functional SUMO family members identified in the human placenta include SUMO-1 to SUMO-3, which are elevated in pre-eclampsia. Whether the fourth isoform,...

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Bibliographic Details
Published in:PloS one 2017-05, Vol.12 (5), p.e0178056-e0178056
Main Authors: Baczyk, Dora, Audette, Melanie C, Drewlo, Sascha, Levytska, Khrystyna, Kingdom, John C
Format: Article
Language:English
Subjects:
Animal models
Arteries
Aspirin
Binding sites
Biochemistry
Biology and life sciences
Birth weight
Blood
Blood flow
Blood pressure
Cell cycle
Cell Hypoxia
Cells, Cultured
Chorioamnionitis
Circulation
Complications
Containers
Control
Deoxyribonucleic acid
Diabetes mellitus
Differentiation
DNA
DNA repair
Edema
Embryonic growth stage
Embryos
Enzymes
Explants
Female
Fetuses
Gene expression
Genomes
Gestation
Glucose
Growth factors
Gynecology
Homology
Humans
Hydrogen peroxide
Hypertension
Hypoxia
Injuries
Isoforms
Kidneys
Liver
Localization
Medicine
Medicine and Health Sciences
Mice
Obstetrics
Oxidation
Oxidative Stress
Placenta
Placenta - metabolism
Placentation
Pre-Eclampsia - genetics
Pre-Eclampsia - metabolism
Preeclampsia
Pregnancy
Pregnancy Proteins - metabolism
Proteins
Regulations
Renal insufficiency
Research and Analysis Methods
Ribonucleic acid
RNA
Rodents
Small Ubiquitin-Related Modifier Proteins - genetics
Small Ubiquitin-Related Modifier Proteins - metabolism
Stem cells
Studies
Sumoylation
Translational research
Trophoblasts - metabolism
Ubiquitin
Visual perception
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Description
Summary:Small ubiquitin-like modifiers (SUMOs) conjugate to proteins post-translationally, thereby affecting target localization, activity and stability. Functional SUMO family members identified in the human placenta include SUMO-1 to SUMO-3, which are elevated in pre-eclampsia. Whether the fourth isoform, SUMO-4, plays a role in placental development and function remains unknown. We tested the hypothesis that SUMO-4 is expressed in the human placenta and demonstrates altered SUMOylation in pre-eclamptic pregnancies. SUMO-4 mRNA (qRT-PCR) and protein (Western blot and immunohistochemistry) were measured in Jar cells, BeWo cells, first trimester placental villous explants and placental tissues across normal gestation and in pre-eclampsia. SUMO-4 expression in response to oxidative stress (H2O2: 0, 0.1, 1 and 5mM), as well as, hypoxia-reperfusion (O2: 1%, 8% and 20%) was measured. Lastly, SUMO-4 binding (covalently vs. non-covalently) to target proteins was investigated. SUMO-4 mRNA and protein were unchanged across gestation. SUMO-4 was present in the villous trophoblast layer throughout gestation. SUMO-4 mRNA expression and protein levels were increased ~2.2-fold and ~1.8-fold in pre-eclamptic placentas compared to age-matched controls, respectively (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0178056