Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models

New innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components-antigens and adjuvants-are crucial for development of modern recombinant TB vaccines. This study describes a new vaccine (GamTBvac) consisting of two mycobacterial antigen fusions (Ag85A...

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Bibliographic Details
Published in:PloS one 2017-04, Vol.12 (4), p.e0176784
Main Authors: Tkachuk, A P, Gushchin, V A, Potapov, V D, Demidenko, A V, Lunin, V G, Gintsburg, A L
Format: Article
Language:English
Subjects:
Accessibility
Active control
Additives
Adjuvants
Adjuvants, Immunologic
Administration, Intravenous
Adults
Aerosols
Alum
Aluminum sulfate
Analysis
Animal models
Animals
Antibodies, Bacterial - blood
Antigen presentation
Antigens
Attenuation
Bacillus Calmette-Guerin vaccine
BCG
BCG Vaccine - immunology
Biodegradability
Biodegradable materials
Biodegradation
Biology and Life Sciences
Biotechnology
Care and treatment
Cell activation
Cell Proliferation - physiology
Chemotherapy
Children
Clinical trials
Cost effectiveness
Degradation
Detoxification
Developing countries
Disease Models, Animal
Dosage and administration
Drug Evaluation, Preclinical
Drug resistance
Emulsions
Epidemiology
Female
Guinea Pigs
HIV
Host-pathogen interactions
Human immunodeficiency virus
Immune response
Immune system
Immunization
Immunization, Secondary
Immunogenicity
Immunogenicity, Vaccine
Immunology
Indicators
Infants
Infections
Interferon
Interleukin 12
Laboratories
LDCs
Lectins
Lung - immunology
Lymph Nodes - immunology
Male
Medicine and Health Sciences
Meningitis
Mice
Mice, Inbred C57BL
Microbiology
Multidrug resistance
Mycobacterium tuberculosis - immunology
Oil
Oxidative stress
Pathogens
Prevention
Proteins
Quality control
Research and Analysis Methods
Safety
Skin
Spleen - immunology
Stimulation
Surgery
Surgical implants
T-Lymphocytes - immunology
Toxicity
Treatment outcome
Tuberculosis
Tuberculosis - immunology
Tuberculosis - prevention & control
Tuberculosis Vaccines - immunology
Vaccines
Vaccines, Subunit - immunology
Vaccines, Synthetic - immunology
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Summary:New innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components-antigens and adjuvants-are crucial for development of modern recombinant TB vaccines. This study describes a new vaccine (GamTBvac) consisting of two mycobacterial antigen fusions (Ag85A and ESAT6-CFP10)-with dextran-binding domain immobilized on dextran and mixed with an adjuvant consisting of DEAE-dextran core, and with CpG oligodeoxynucleotides (TLR9 agonists). GamTBvac and its components were assessed for immunogenicity and protective efficacy in GamTBvac-prime/boost and BCG-prime/ GamTBvac-boost in murine and guinea pig TB models. Results show that in both infectious models, GamTBvac has a strong immunogenicity and significant protective effect against Mycobacterium tuberculosis strain H37Rv under aerosol and intravenous challenges. GamTBvac showed a particularly strong protective effect as a BCG booster vaccine.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0176784