KIAA1199 promotes migration and invasion by Wnt/β-catenin pathway and MMPs mediated EMT progression and serves as a poor prognosis marker in gastric cancer

KIAA1199 was upregulated in diverse cancers, but the association of KIAA1199 with gastric cancer (GC), the biological role of KIAA1199 in GC cells and the related molecular mechanisms remain to be elucidated. KIAA1199 expression was analysed by reverse transcription-polymerase chain reaction assay (...

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Bibliographic Details
Published in:PloS one 2017-04, Vol.12 (4), p.e0175058
Main Authors: Jia, Shuqin, Qu, Tingting, Wang, Xiaohong, Feng, Mengmeng, Yang, Yang, Feng, Xuemin, Ma, Ruiting, Li, Wenmei, Hu, Ying, Feng, Yi, Ji, Ke, Li, Ziyu, Jiang, Wenguo, Ji, Jiafu
Format: Article
Language:English
Subjects:
Aged
beta Catenin - genetics
beta Catenin - metabolism
Biology and Life Sciences
Biomarkers
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Breast cancer
Cancer
Cancer therapies
Cell adhesion & migration
Cell growth
Cell Line, Tumor
Cell Movement
Cell Proliferation
Chemotherapy
Colorectal cancer
Education
Enzymatic activity
Epithelial-Mesenchymal Transition - genetics
Female
Gastric cancer
Gastrointestinal surgery
Gene Expression Regulation, Neoplastic
Genotype & phenotype
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
Laboratories
Male
Matrix Metalloproteinase 2 - genetics
Matrix Metalloproteinase 2 - metabolism
Medical prognosis
Medicine and Health Sciences
Metastasis
Middle Aged
Neoplasm Invasiveness
Neoplasm Staging
Oncogenes
Patients
Polymerase chain reaction
Prognosis
Proteins
Proteins - antagonists & inhibitors
Proteins - genetics
Proteins - metabolism
Research and analysis methods
Ribonucleic acid
RNA
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
Signal transduction
Stomach cancer
Stomach Neoplasms - diagnosis
Stomach Neoplasms - genetics
Stomach Neoplasms - mortality
Stomach Neoplasms - pathology
Survival Analysis
Wnt Signaling Pathway
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Summary:KIAA1199 was upregulated in diverse cancers, but the association of KIAA1199 with gastric cancer (GC), the biological role of KIAA1199 in GC cells and the related molecular mechanisms remain to be elucidated. KIAA1199 expression was analysed by reverse transcription-polymerase chain reaction assay (RT-PCR) and immunohistochemistry (IHC) in GC patient tissue. The small hairpin RNA (shRNA) was applied for the knockdown of endogenous KIAA1199 in NCI-N87 and AGS cells. MTT, colony formation, scratch wounding migration, transwell chamber migration and invasion assays were employed respectively to investigate the role of KIAA1199 in GC cells. The potential signaling pathway of KIAA1199 induced migration and invasion was detected. KIAA1199 was upregulated in GC tissue and was an essential independent marker for poor prognosis. Knockdown KIAA1199 suppressed the proliferation, migration and invasion in GC cells. KIAA1199 stimulated the Wnt/β-catenin signaling pathway and the enzymatic activity of matrix metalloproteinase (MMP) family members and thus accelerated the epithelial-to-mesenchymal transition (EMT) progression in GC cells. These findings demonstrated that KIAA1199 was upregulated in GC tissue and associated with worse clinical outcomes in GC, and KIAA1199 acted as an oncogene by promoting migration and invasion through the enhancement of Wnt/β-catenin signaling pathway and MMPs mediated EMT progression in GC cells.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0175058