Hydrophobic pinning with copper nanowhiskers leads to bactericidal properties

The considerable morbidity associated with hospitalized patients and clinics in developed countries due to biofilm formation on biomedical implants and surgical instruments is a heavy economic burden. An alternative to chemically treated surfaces for bactericidal activity started emerging from micro...

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Bibliographic Details
Published in:PloS one 2017-04, Vol.12 (4), p.e0175428-e0175428
Main Authors: Singh, Ajay Vikram, Baylan, Semanur, Park, Byung-Wook, Richter, Gunther, Sitti, Metin
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Bactericidal activity
Biofilms
Biofilms - drug effects
Biology and Life Sciences
Biomedical materials
Carbon
Cell culture
Cell death
Cell density
Copper
Copper - chemistry
Copper - pharmacology
Electron microscopy
Engineering
Engineering and Technology
Extracellular matrix
Health risks
Hydrophobic and Hydrophilic Interactions
Hydrophobic effect
Hydrophobicity
Intelligence
Leaching
Medical devices
Medical equipment
Medicine and Health Sciences
Microbial mats
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Molecular beam epitaxy
Molecular structure
Morbidity
Morphology
Pharmaceuticals
Physical Sciences
Physiological aspects
Properties
Proteins
Research and Analysis Methods
Scanning electron microscopy
Staphylococcus infections
Studies
Surface Properties
Thin films
Topography
Transplants & implants
Water droplets
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Summary:The considerable morbidity associated with hospitalized patients and clinics in developed countries due to biofilm formation on biomedical implants and surgical instruments is a heavy economic burden. An alternative to chemically treated surfaces for bactericidal activity started emerging from micro/nanoscale topographical cues in the last decade. Here, we demonstrate a putative antibacterial surface using copper nanowhiskers deposited by molecular beam epitaxy. Furthermore, the control of biological response is based on hydrophobic pinning of water droplets in the Wenzel regime, causing mechanical injury and cell death. Scanning electron microscopy images revealed the details of the surface morphology and non-contact mode laser scanning of the surface revealed the microtopography-associated quantitative parameters. Introducing the bacterial culture over nanowhiskers produces mechanical injury to cells, leading to a reduction in cell density over time due to local pinning of culture medium to whisker surfaces. Extended culture to 72 hours to observe biofilm formation revealed biofilm inhibition with scattered microcolonies and significantly reduced biovolume on nanowhiskers. Therefore, surfaces patterned with copper nanowhiskers can serve as potential antibiofilm surfaces. The topography-based antibacterial surfaces introduce a novel prospect in developing mechanoresponsive nanobiomaterials to reduce the risk of medical device biofilm-associated infections, contrary to chemical leaching of copper as a traditional bactericidal agent.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0175428