The gut microbiota of insecticide-resistant insects houses insecticide-degrading bacteria: A potential source for biotechnological exploitation

The exploration of new niches for microorganisms capable of degrading recalcitrant molecules is still required. We hypothesized the gut microbiota associated with insect-resistant lines carry pesticide degrading bacteria, and predicted they carry bacteria selected to degrade pesticides they were res...

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Bibliographic Details
Published in:PloS one 2017-03, Vol.12 (3), p.e0174754-e0174754
Main Authors: Almeida, Luis Gustavo de, Moraes, Luiz Alberto Beraldo de, Trigo, José Roberto, Omoto, Celso, Cônsoli, Fernando Luis
Format: Article
Language:English
Subjects:
Agrochemicals
Animals
Bacteria
Bacteria - drug effects
Benzamides - pharmacology
Biodegradation
Biology and Life Sciences
Bioremediation
Biotechnology
Chlorpyrifos
Degradation
Deltamethrin
Drug Combinations
Ecology
Evolution
Exploitation
Gas Chromatography-Mass Spectrometry
Gastrointestinal Microbiome - physiology
Host-parasite interactions
Housing
Insecticide resistance
Insecticides
Insecticides - pharmacology
Insects
Instars
Intestinal microflora
Laboratories
Larvae
Macrolides - pharmacology
Medicine and Health Sciences
Microbiota (Symbiotic organisms)
Microorganisms
Nitriles - pharmacology
Pest control
Pesticide resistance
Pesticides
Pyrethrins - pharmacology
Research and Analysis Methods
Residential areas
Selective media
Spodoptera - microbiology
Spodoptera frugiperda
Tandem Mass Spectrometry
Trends
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Summary:The exploration of new niches for microorganisms capable of degrading recalcitrant molecules is still required. We hypothesized the gut microbiota associated with insect-resistant lines carry pesticide degrading bacteria, and predicted they carry bacteria selected to degrade pesticides they were resistant to. We isolated and accessed the pesticide-degrading capacity of gut bacteria from the gut of fifth instars of Spodoptera frugiperda strains resistant to lambda-cyhalothrin, deltamethrin, chlorpyrifos ethyl, spinosad and lufenuron, using insecticide-selective media. Sixteen isolates belonging to 10 phylotypes were obtained, from which four were also associated with the susceptible strain. However, growth of gut bacteria associated with larvae from the susceptible strain was not obtained in any of the insecticide-based selective media tested. Growth of isolates was affected by the concentration of insecticides in the media, and all grew well up to 40 μg/ml. The insecticide-degrading capacity of selected isolates was assessed by GC or LC-MS/MS analyses. In conclusion, resistant strains of S. frugiperda are an excellent reservoir of insecticide-degrading bacteria with bioremediation potential. Moreover, gut-associated bacteria are subjected to the selection pressure imposed by insecticides on their hosts and may influence the metabolization of pesticides in insects.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0174754