Chitosan-assisted differentiation of porcine adipose tissue-derived stem cells into glucose-responsive insulin-secreting clusters

The unique advantage of easy access and abundance make the adipose-derived stem cells (ADSCs) a promising system of multipotent cells for transplantation and regenerative medicine. Among the available sources, porcine ADSCs (pADSCs) deserve especial attention due to the close resemblance of human an...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2017-03, Vol.12 (3), p.e0172922-e0172922
Main Authors: Liu, Hui-Yu, Chen, Chih-Chien, Lin, Yuan-Yu, Chen, Yu-Jen, Liu, Bing-Hsien, Wong, Shiu-Chung, Wu, Cheng-Yu, Chang, Yun-Tsui, Chou, Han-Yi E, Ding, Shih-Torng
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose Tissue - cytology
Adipose Tissue - drug effects
Animal models
Animal sciences
Animals
Biology and Life Sciences
Biomedical materials
Biotechnology
Body fat
Bone marrow
Cell culture
Cell Differentiation - drug effects
Chitin
Chitosan
Chitosan - pharmacology
Clusters
Culture Media
Diabetes
Diabetes mellitus
Diabetes mellitus (insulin dependent)
Differentiation
Dosage
Enzyme-Linked Immunosorbent Assay
Fibroblasts
Glucose
Growth
Growth factors
Hogs
Homeobox
Innovations
Insulin
Insulin - metabolism
Insulin Secretion
Insurance claims
Medicine
Medicine and Health Sciences
Metabolism
Pancreas
Physical Sciences
Properties
Proteins
Regenerative medicine
Research and Analysis Methods
Rodents
Stem cell transplantation
Stem cells
Stem Cells - cytology
Stem Cells - drug effects
Subcutaneous Fat - cytology
Swine
Tissue engineering
Transplantation
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The unique advantage of easy access and abundance make the adipose-derived stem cells (ADSCs) a promising system of multipotent cells for transplantation and regenerative medicine. Among the available sources, porcine ADSCs (pADSCs) deserve especial attention due to the close resemblance of human and porcine physiology, as well as for the upcoming availability of humanized porcine models. Here, we report on the isolation and conversion of pADSCs into glucose-responsive insulin-secreting cells. We used the stromal-vascular fraction of the dorsal subcutaneous adipose from 9-day-old male piglets to isolate pADSCs, and subjected the cells to an induction scheme for differentiation on chitosan-coated plates. This one-step procedure promoted differentiation of pADSCs into pancreatic islet-like clusters (PILC) that are characterized by the expression of a repertoire of pancreatic proteins, including pancreatic and duodenal homeobox (Pdx-1), insulin gene enhancer protein (ISL-1) and insulin. Upon glucose challenge, these PILC secreted high amounts of insulin in a dose-dependent manner. Our data also suggest that chitosan plays roles not only to enhance the differentiation potential of pADSCs, but also to increase the glucose responsiveness of PILCs. Our novel approach is, therefore, of great potential for transplantation-based amelioration of type 1 diabetes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0172922