Polymorphisms in HTR2A and DRD4 Predispose to Smoking and Smoking Quantity

Genes encoding the receptors involved in the dopaminergic and serotonergic pathways are potential candidates in the mechanisms of nicotine addiction. To identify genetic variants in the promoter regions and exons of the DRD4 and HTR2A genes associated with tobacco smoking and the degree of nicotine...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2017-01, Vol.12 (1), p.e0170019-e0170019
Main Authors: Pérez-Rubio, Gloria, Ramírez-Venegas, Alejandra, Noé Díaz, Valeri, García Gómez, Leonor, Elvira Fabián, Karina, García Carmona, Salvador, López-Flores, Luis A, Ambrocio-Ortiz, Enrique, Contreras Romero, Rocío, Alcantar-Ayala, Noé, Sansores, Raúl H, Falfán-Valencia, Ramcés
Format: Article
Language:English
Subjects:
Addictions
Aged
Alleles
Biology and Life Sciences
Case-Control Studies
Cigarette smoking
Cigarettes
Compounds
Cross-Sectional Studies
Deoxyribonucleic acid
Disease
DNA
DNA methylation
Dopamine
Dopamine D4 receptors
Dopamine receptors
Exons
Genes
Genetic aspects
Genetic Association Studies
Genetic diversity
Genetic variance
Genotyping
Haplotypes
Health aspects
Humans
Male
Medicine and Health Sciences
Mexico - epidemiology
Middle Aged
Nicotine
People and Places
Polymorphism, Single Nucleotide - genetics
Polymorphism, Single Nucleotide - physiology
Population
Public health
Real-Time Polymerase Chain Reaction
Receptor, Serotonin, 5-HT2A - genetics
Receptors
Receptors, Dopamine D4 - genetics
Serotonin
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Smokers
Smoking
Smoking - epidemiology
Smoking - genetics
Smoking cessation
Social Sciences
Studies
Tobacco
Tobacco smoking
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genes encoding the receptors involved in the dopaminergic and serotonergic pathways are potential candidates in the mechanisms of nicotine addiction. To identify genetic variants in the promoter regions and exons of the DRD4 and HTR2A genes associated with tobacco smoking and the degree of nicotine addiction in Mexican mestizos. The study included 438 non-smokers (NS) and 1,157 current smokers, ranked based on their consumption of cigarettes per day (cpd): 574 heavy smokers (HS, >20 cpd) and 583 light smokers (LS, 1-10 cpd). Genotyping was performed for 4 and 8 single nucleotide polymorphisms (SNPs) in the DRD4 and HTR2A genes, respectively. The C allele of rs1800955 in DRD4 was found to be associated with cigarette smoking in the HS vs. NS and LS vs. NS comparisons (p = 2.34E-03 and p = 1.13E-03, respectively); the association was maintained in the homozygous CC genotype (p = 5.00E-04 and p = 2.00E-04, respectively). The T allele of rs6313 in HTR2A was significantly associated with cigarette smoking and a greater degree of nicotine addiction (p = 4.77E-03, OR = 1.55); the association was maintained in the homozygous genotype (TT) (p = 4.90E-03, OR = 1.96). The A allele of rs6313 was associated with cigarette smoking in the HS vs. NS comparison (p = 1.53E-02, OR = 1.36); the risk was nearly doubled in the homozygous AA genotype (p = 1.30E-03, OR = 1.83) compared with the heterozygous GA genotype (OR = 1.38). Among Mexican mestizos, the C allele of rs1800955 in the DRD4 gene and the A allele of rs6311 in the HTR2A gene are associated with cigarette smoking, whereas the T allele of rs6313 in HTR2A is associated with cigarette smoking and the degree of nicotine addiction.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0170019