White - cGMP Interaction Promotes Fast Locomotor Recovery from Anoxia in Adult Drosophila

Increasing evidence indicates that the white (w) gene in Drosophila possesses extra-retinal functions in addition to its classical role in eye pigmentation. We have previously shown that w+ promotes fast and consistent locomotor recovery from anoxia, but how w+ modulates locomotor recovery is largel...

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Bibliographic Details
Published in:PloS one 2017-01, Vol.12 (1), p.e0168361-e0168361
Main Authors: Xiao, Chengfeng, Robertson, R Meldrum
Format: Article
Language:English
Subjects:
Animals
Anoxia
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette Transporters - metabolism
Biology and Life Sciences
Circadian rhythm
Cyclic GMP
Cyclic GMP - metabolism
Cyclic guanosine monophosphate
Cyclic Nucleotide Phosphodiesterases, Type 1 - genetics
Cyclic Nucleotide Phosphodiesterases, Type 1 - metabolism
Data recovery
Drosophila
Drosophila - genetics
Drosophila - metabolism
Drosophila melanogaster
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Eye Proteins - genetics
Eye Proteins - metabolism
Feeding
Female
Gene Expression Regulation
Genes
Genetic aspects
Guanosine
Guanylate cyclase
Insects
Kinases
Laboratories
Light
Locomotion - genetics
Locomotion - radiation effects
Male
Medicine and Health Sciences
Mutants
Mutation
Oxygen - metabolism
Photoreceptors
Physiological aspects
Pigmentation
Proteins
Recovery (Medical)
Research and Analysis Methods
Retina
RNA-mediated interference
Signal transduction
Sildenafil
Variation
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Summary:Increasing evidence indicates that the white (w) gene in Drosophila possesses extra-retinal functions in addition to its classical role in eye pigmentation. We have previously shown that w+ promotes fast and consistent locomotor recovery from anoxia, but how w+ modulates locomotor recovery is largely unknown. Here we show that in the absence of w+, several PDE mutants, especially cyclic guanosine monophosphate (cGMP)-specific PDE mutants, display wildtype-like fast locomotor recovery from anoxia, and that during the night time, locomotor recovery was light-sensitive in white-eyed mutant w1118, and light-insensitive in PDE mutants under w1118 background. Data indicate the involvement of cGMP in the modulation of recovery timing and presumably, light-evoked cGMP fluctuation is associated with light sensitivity of locomotor recovery. This was further supported by the observations that w-RNAi-induced delay of locomotor recovery was completely eliminated by upregulation of cGMP through multiple approaches, including PDE mutation, simultaneous overexpression of an atypical soluble guanylyl cyclase Gyc88E, or sildenafil feeding. Lastly, prolonged sildenafil feeding promoted fast locomotor recovery from anoxia in w1118. Taken together, these data suggest that a White-cGMP interaction modulates the timing of locomotor recovery from anoxia.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0168361