Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1α-Hydroxylase by the Fetal-Maternal Interface

Effects of vitamin D deficiency in pregnancy have been associated with some adverse pregnancy outcomes. The 25-hydroxyvitamin D3-1α-hydroxylase (CYP27B1) is integral to the vitamin D metabolic pathway. The enzyme catalyzes localized conversion of pro-hormone 25-hydroxyvitamin D3 to active 1,25-dihyd...

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Published in:PloS one 2016-12, Vol.11 (12), p.e0165589-e0165589
Main Authors: Wang, Li-Qin, Yan, Xiao-Ting, Yan, Chun-Fang, Zhang, Xin-Wen, Hui, Ling-Yun, Xue, Mingzhan, Yu, Xue-Wen
Format: Article
Language:English
Subjects:
25-Hydroxyvitamin D3 1-alpha-Hydroxylase - biosynthesis
25-Hydroxyvitamin D3 1-alpha-Hydroxylase - genetics
25-Hydroxyvitamin D3 1-alpha-Hydroxylase - metabolism
Abortion
Abortion, Habitual - pathology
Adult
Age
Autoimmune diseases
Biology and Life Sciences
Calcitriol
Calcitriol - biosynthesis
Chorionic Villi - metabolism
Confocal microscopy
Cytokines
Decidua
Decidua - metabolism
Dihydroxyvitamin D3
Enzymes
Epithelial cells
Female
Fetuses
Gene expression
Gynecology
Hospitals
Humans
Hydroxylase
Immunohistochemistry
In vitro fertilization
Interferon
Interferon-gamma - metabolism
Interleukin 10
Interleukin 2
Interleukin-10 - metabolism
Interleukin-2 - metabolism
Localization
Maternal effects
Medicine and Health Sciences
Menstruation
Microscopy
Miscarriage
mRNA
Nutrient deficiency
Obstetrics
Physical sciences
Pregnancy
Research and Analysis Methods
RNA, Messenger - biosynthesis
Tissues
Trophoblasts
Tumor Necrosis Factor-alpha - metabolism
Tumor necrosis factor-TNF
Tumor necrosis factor-α
Ultrasonic imaging
Uterine Hemorrhage - pathology
Villus
Vitamin D
Vitamin D Deficiency - pathology
Vitamin D3
Vitamin deficiency
Womens health
γ-Interferon
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Summary:Effects of vitamin D deficiency in pregnancy have been associated with some adverse pregnancy outcomes. The 25-hydroxyvitamin D3-1α-hydroxylase (CYP27B1) is integral to the vitamin D metabolic pathway. The enzyme catalyzes localized conversion of pro-hormone 25-hydroxyvitamin D3 to active 1,25-dihydroxyvitamin D3. Our aim was to investigate the expression of CYP27B1 at the fetal-maternal interface in the first trimester pregnancy and to determine whether CYP27B1 was associated with recurrent miscarriage (RM). Expressions of CYP27B1 mRNA and protein in villi and decidua from 20 women undergoing primary miscarriage, 20 women with RM and 20 women with normal pregnancy were evaluated by western blot, and quantitative real-time PCR. The co-localization of CYP27B1 and certain cytokines including IL-10, IFN-γ, TNF-α, and IL-2 expression were examined using immunohistochemistry and confocal microscopy. Women with RM had a significantly lower expression of CYP27B1 mRNA and protein in villous and decidual tissues compared with the normal pregnant women (P = 0.000 in villus, P = 0.002 in decidua for mRNA; P = 0.036 in villus, P = 0.007 in decidua for protein.). Compared with the normal pregnancy, immunostaining for CYP27B1 was significantly decreased in villous trophoblasts and decidual glandular epithelial cells in RM women. No significant differences in the localization of CYP27B1, IL-10, IFN-γ, TNF-α, and IL-2 expression were identified between the normal pregnant and RM women. Women with RM have a lower level of CYP27B1 expression in chorionic villi and decidua compared with normal pregnant women, suggesting that reduced CYP27B1 expression may be associated with RM. The consistent localization of CYP27B1 and IL-10, IFN-γ, TNF-α, and IL-2 expression in villous and decidual tissues suggests the importance of the local production of 1,25(OH)2D3 at the fetal-maternal interface to regulate cytokine responses.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0165589