Precursor B Cells Increase in the Lung during Airway Allergic Inflammation: A Role for B Cell-Activating Factor

B cells, key cells in allergic inflammation, differentiate in the bone marrow and their precursors include pro-B, pre-B and immature B cells. Eosinophil progenitor cells increase in the lung after allergen exposure. However, the existence and possible role of B cell precursors in the lung during all...

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Bibliographic Details
Published in:PloS one 2016-08, Vol.11 (8), p.e0161161-e0161161
Main Authors: Samitas, Konstantinos, Malmhäll, Carina, Rådinger, Madeleine, Ramos-Ramirez, Patricia, Lu, You, Deák, Tünde, Semitekolou, Maria, Gaga, Mina, Sjöstrand, Margareta, Lötvall, Jan, Bossios, Apostolos
Format: Article
Language:English
Subjects:
Allergens
Allergies
Alveoli
Analysis
Animals
Apoptosis
Apoptosis - immunology
Asthma
Asthma - immunology
Asthma - metabolism
Asthma - pathology
B cells
B-Cell Activating Factor - metabolism
Bax protein
Biology
Biology and Life Sciences
Biomedical research
BLyS protein
Bone marrow
Bronchial Hyperreactivity - immunology
Bronchial Hyperreactivity - metabolism
Bronchial Hyperreactivity - pathology
Bronchoalveolar Lavage Fluid - immunology
Bronchus
Care and treatment
Case-Control Studies
ccr10 expression
CD40 antigen
CD86 antigen
Cells (biology)
Chemotaxis
Clinical Medicine
Colonies
CXCR4 protein
Cytokines
Cytometry
differentiation
eosinophil progenitors
Eosinophils
Eosinophils - immunology
Eosinophils - metabolism
Eosinophils - pathology
Exposure
factor baff
Female
Flow Cytometry
Humans
Hypersensitivity
Immunoglobulins
Immunology
In vivo methods and tests
Inflammation
Interleukin 7
Internal medicine
Klinisk medicin
Laboratories
Leukocytes (eosinophilic)
Lungs
Lymphocyte receptors
Lymphocytes B
Male
Medicin och hälsovetenskap
Medicine
Medicine and Health Sciences
Mice
Mice, Inbred BALB C
Middle Aged
Nutrition
Osteoprogenitor cells
Pneumonia - immunology
Pneumonia - metabolism
Pneumonia - pathology
Precursor Cells, B-Lymphoid - immunology
Precursor Cells, B-Lymphoid - metabolism
Precursor Cells, B-Lymphoid - pathology
Precursors
proliferation
Receptors
Research and Analysis Methods
Respiratory tract
Respiratory tract diseases
Risk factors
Science & Technology - Other Topics
secreting cells
Signal Transduction
Stem cells
survival
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Summary:B cells, key cells in allergic inflammation, differentiate in the bone marrow and their precursors include pro-B, pre-B and immature B cells. Eosinophil progenitor cells increase in the lung after allergen exposure. However, the existence and possible role of B cell precursors in the lung during allergic inflammation remains elusive. A BALB/c mouse model of allergic airway inflammation was utilized to perform phenotypic and quantification analyses of pro-B and pre-B cells in the lung by flow cytometry. B cell maturation factors IL-7 and B cell-activating factor (BAFF) and their receptors (CD127 and BAFFR, BCMA, TACI, respectively) were also evaluated in the lung and serum. The effect of anti-BAFF treatment was investigated both in vivo (i.p. administration of BAFF-R-Ig fusion protein) and in vitro (colony forming cell assay). Finally, BAFF levels were examined in the bronchoalveolar lavage (BAL) of asthmatic patients and healthy controls. Precursor pro and pre-B cells increase in the lung after allergen exposure, proliferate in the lung tissue in vivo, express markers of chemotaxis (CCR10 and CXCR4) and co-stimulation (CD40, CD86) and are resistant to apoptosis (Bax). Precursor B cells express receptors for BAFF at baseline, while after allergen challenge both their ligand BAFF and the BCMA receptor expression increases in B cell precursors. Blocking BAFFR in the lung in vivo decreases eosinophils and proliferating precursor B cells. Blocking BAFFR in bone marrow cultures in vitro reduces pre-B colony formation units. BAFF is increased in the BAL of severe asthmatics. Our data support the concept of a BAFF-mediated role for B cell precursors in allergic airway inflammation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0161161