Genome Sequence of the Edible Cultivated Mushroom Lentinula edodes (Shiitake) Reveals Insights into Lignocellulose Degradation

Lentinula edodes, one of the most popular, edible mushroom species with a high content of proteins and polysaccharides as well as unique aroma, is widely cultivated in many Asian countries, especially in China, Japan and Korea. As a white rot fungus with lignocellulose degradation ability, L. edodes...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2016-08, Vol.11 (8), p.e0160336-e0160336
Main Authors: Chen, Lianfu, Gong, Yuhua, Cai, Yingli, Liu, Wei, Zhou, Yan, Xiao, Yang, Xu, Zhangyi, Liu, Yin, Lei, Xiaoyu, Wang, Gangzheng, Guo, Mengpei, Ma, Xiaolong, Bian, Yinbing
Format: Article
Language:English
Subjects:
Acids
Agricultural resources
Agricultural wastes
Agriculture
Analysis
Aroma
Biodegradation
Bioinformatics
Biological evolution
Biology and Life Sciences
Carbohydrates
Cellulose
Cladistic analysis
Cultivation
Degradation
Divergence
Enzymes
Evolution
Evolution (Biology)
Food science
Fungal Proteins - genetics
Fungi
Gene expression
Genes
Genetic aspects
Genetic research
Genome, Fungal
Genomes
Genomics
Glucose
Gymnopus
High-Throughput Nucleotide Sequencing
Laboratories
Lentinula edodes
Lignin
Lignin - genetics
Lignin - metabolism
Lignocellulose
Molecular biology
Mushrooms
Mycelia
Nucleotide sequence
Phylogenetics
Phylogeny
Physical Sciences
Plant sciences
Polysaccharides
Proteins
Research and Analysis Methods
Saccharides
Sawdust
Shiitake Mushrooms - genetics
Shiitake Mushrooms - growth & development
Shiitake Mushrooms - metabolism
Species
Straw
Transcription (Genetics)
White rot
White rot fungi
Wood
Wood waste
Wood wastes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lentinula edodes, one of the most popular, edible mushroom species with a high content of proteins and polysaccharides as well as unique aroma, is widely cultivated in many Asian countries, especially in China, Japan and Korea. As a white rot fungus with lignocellulose degradation ability, L. edodes has the potential for application in the utilization of agriculture straw resources. Here, we report its 41.8-Mb genome, encoding 14,889 predicted genes. Through a phylogenetic analysis with model species of fungi, the evolutionary divergence time of L. edodes and Gymnopus luxurians was estimated to be 39 MYA. The carbohydrate-active enzyme genes in L. edodes were compared with those of the other 25 fungal species, and 101 lignocellulolytic enzymes were identified in L. edodes, similar to other white rot fungi. Transcriptome analysis showed that the expression of genes encoding two cellulases and 16 transcription factor was up-regulated when mycelia were cultivated for 120 minutes in cellulose medium versus glucose medium. Our results will foster a better understanding of the molecular mechanism of lignocellulose degradation and provide the basis for partial replacement of wood sawdust with agricultural wastes in L. edodes cultivation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0160336