Association of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ossification of the Posterior Longitudinal Ligament of the Cervical Spine

Ossification of the posterior longitudinal ligament (OPLL) of the spine is considered a multifactorial and polygenic disease. We aimed to investigate the association between four single nucleotide polymorphisms (SNPs) of pre-miRNAs [miR-146aC>G (rs2910164), miR-149T>C (rs2292832), miR-196a2T&g...

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Published in:PloS one 2016-07, Vol.11 (7), p.e0159756-e0159756
Main Authors: Lim, Jae Joon, Shin, Dong Ah, Jeon, Young Joo, Kumar, Hemant, Sohn, Seil, Min, Hyoung Sik, Lee, Jang Bo, Kuh, Sung Uk, Kim, Keung Nyun, Kim, Jung Oh, Kim, Ok Joon, Ropper, Alexander E, Kim, Nam Keun, Han, In Bo
Format: Article
Language:English
Subjects:
Adult
Aged
Alleles
Analysis
Asian Continental Ancestry Group - genetics
Bioinformatics
Biology and life sciences
Bone density
Calcification
Case-Control Studies
Cervical Vertebrae
Confidence intervals
Disease
Female
Gene expression
Gene Frequency
Gene polymorphism
Genetic aspects
Genetic Predisposition to Disease
Genomes
Genomics
Genotype
Genotypes
Haplotypes
Hospitals
Humans
Inflammation
Influence
Life sciences
Ligaments
Male
Medicine and Health Sciences
Metabolism
MicroRNA
MicroRNAs
MicroRNAs - chemistry
MicroRNAs - genetics
Middle Aged
Neurosurgery
NMR
Nuclear magnetic resonance
Ossification
Ossification of Posterior Longitudinal Ligament - genetics
Osteoarthritis
Pathogenesis
Patients
Polymerase chain reaction
Polymorphism
Polymorphism, Single Nucleotide
Republic of Korea
Restriction fragment length polymorphism
Risk factors
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Spine
Spine (cervical)
Studies
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Summary:Ossification of the posterior longitudinal ligament (OPLL) of the spine is considered a multifactorial and polygenic disease. We aimed to investigate the association between four single nucleotide polymorphisms (SNPs) of pre-miRNAs [miR-146aC>G (rs2910164), miR-149T>C (rs2292832), miR-196a2T>C (rs11614913), and miR-499A>G (rs3746444)] and the risk of cervical OPLL in the Korean population. The genotypic frequencies of these four SNPs were analyzed in 207 OPLL patients and 200 controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. For four SNPs in pre-miRNAs, no significant differences were found between OPLL patients and controls. However, subgroup analysis based on OPLL subgroup (continuous: continuous type plus mixed type, segmental: segmental and localized type) showed that miR-499GG genotype was associated with an increased risk of segmental type OPLL (adjusted odds ratio = 4.314 with 95% confidence interval: 1.109-16.78). In addition, some allele combinations (C-T-T-G, G-T-T-A, and G-T-C-G of miR-146a/-149/-196a2/-499) and combined genotypes (miR-149TC/miR-196a2TT) were associated with increased OPLL risk, whereas the G-T-T-G and G-C-C-G allele combinations were associated with decreased OPLL risk. The results indicate that GG genotype of miR-499 is associated with significantly higher risks of OPLL in the segmental OPLL group. The miR-146a/-149/-196a2/-499 allele combinations may be a genetic risk factor for cervical OPLL in the Korean population.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0159756