Identification and Validation of SNP Markers Linked to Dwarf Traits Using SLAF-Seq Technology in Lagerstroemia

The genetic control of plant architecture is a promising approach to breed desirable cultivars, particularly in ornamental flowers. In this study, the F1 population (142 seedlings) derived from Lagerstroemia fauriei (non-dwarf) × L. indica 'Pocomoke' (dwarf) was phenotyped for six traits (...

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Bibliographic Details
Published in:PloS one 2016-07, Vol.11 (7), p.e0158970-e0158970
Main Authors: Ye, Yuanjun, Cai, Ming, Ju, Yiqian, Jiao, Yao, Feng, Lu, Pan, Huitang, Cheng, Tangren, Zhang, Qixiang
Format: Article
Language:English
Subjects:
Architectural engineering
Biology and Life Sciences
Brassica napus
Correlation
Cultivars
Engineering research
Flowers
Genetic aspects
Genetic control
Genetic Markers - genetics
Genomes
Genomics
Genotype
Genotype & phenotype
Germplasm
High-Throughput Nucleotide Sequencing
Laboratories
Lagerstroemia
Lagerstroemia - anatomy & histology
Lagerstroemia - genetics
Lagerstroemia - growth & development
Landscape architecture
Marker-assisted selection
Markers
Oryza sativa
Personality traits
Phenotype
Physiological aspects
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Population genetics
Population studies
Predictability
Research and Analysis Methods
Rice
Seedlings
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Sorghum
Technology
Technology utilization
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Summary:The genetic control of plant architecture is a promising approach to breed desirable cultivars, particularly in ornamental flowers. In this study, the F1 population (142 seedlings) derived from Lagerstroemia fauriei (non-dwarf) × L. indica 'Pocomoke' (dwarf) was phenotyped for six traits (plant height (PH), internode length (IL), internode number, primary lateral branch height (PLBH), secondary lateral branch height and primary branch number), and the IL and PLBH traits were positively correlated with the PH trait and considered representative indexes of PH. Fifty non-dwarf and dwarf seedlings were pooled and subjected to a specific-locus amplified fragment sequencing (SLAF-seq) method, which screened 1221 polymorphic markers. A total of 3 markers segregating between bulks were validated in the F1 population, with the M16337 and M38412 markers highly correlated with the IL trait and the M25207 marker highly correlated with the PLBH trait. These markers provide a predictability of approximately 80% using a single marker (M25207) and a predictability of 90% using marker combinations (M16337 + M25207) in the F1 population, which revealed that the IL and the PLBH traits, especially the PLBH, were the decisive elements for PH in terms of molecular regulation. Further validation was performed in the BC1 population and a set of 28 Lagerstroemia stocks using allele-specific PCR (AS-PCR) technology, and the results showed the stability and reliability of the SNP markers and the co-determination of PH by multiple genes. Our findings provide an important theoretical and practical basis for the early prediction and indirect selection of PH using the IL and the PLBH, and the detected SNPs may be useful for marker-assisted selection (MAS) in crape myrtle.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0158970