Mitochondrial Polyadenylation Is a One-Step Process Required for mRNA Integrity and tRNA Maturation

Polyadenylation has well characterised roles in RNA turnover and translation in a variety of biological systems. While polyadenylation on mitochondrial transcripts has been suggested to be a two-step process required to complete translational stop codons, its involvement in mitochondrial RNA turnove...

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Bibliographic Details
Published in:PLoS genetics 2016-05, Vol.12 (5), p.e1006028-e1006028
Main Authors: Bratic, Ana, Clemente, Paula, Calvo-Garrido, Javier, Maffezzini, Camilla, Felser, Andrea, Wibom, Rolf, Wedell, Anna, Freyer, Christoph, Wredenberg, Anna
Format: Article
Language:English
Subjects:
Animals
Biology and Life Sciences
Codon, Terminator
Councils
Crystal structure
Drosophila melanogaster
Drosophila melanogaster - genetics
Experiments
Gene expression
Gene Knockdown Techniques
Genomes
Insects
Medicin och hälsovetenskap
Messenger RNA
Methods
Mitochondria
Mitochondria - genetics
Mitochondrial DNA
Mitochondrial Proteins - biosynthesis
Mitochondrial Proteins - genetics
Physiological aspects
Polyadenylation - genetics
Protein Biosynthesis - genetics
Proteins
Research and Analysis Methods
RNA polymerase
RNA sequencing
RNA, Messenger - genetics
RNA, Transfer - genetics
Transfer RNA
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Summary:Polyadenylation has well characterised roles in RNA turnover and translation in a variety of biological systems. While polyadenylation on mitochondrial transcripts has been suggested to be a two-step process required to complete translational stop codons, its involvement in mitochondrial RNA turnover is less well understood. We studied knockdown and knockout models of the mitochondrial poly(A) polymerase (MTPAP) in Drosophila melanogaster and demonstrate that polyadenylation of mitochondrial mRNAs is exclusively performed by MTPAP. Further, our results show that mitochondrial polyadenylation does not regulate mRNA stability but protects the 3' terminal integrity, and that despite a lack of functioning 3' ends, these trimmed transcripts are translated, suggesting that polyadenylation is not required for mitochondrial translation. Additionally, loss of MTPAP leads to reduced steady-state levels and disturbed maturation of tRNACys, indicating that polyadenylation in mitochondria might be important for the stability and maturation of specific tRNAs.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1006028