Comprehensive Analysis of ABA Effects on Ethylene Biosynthesis and Signaling during Tomato Fruit Ripening

ABA has been widely acknowledged to regulate ethylene biosynthesis and signaling during fruit ripening, but the molecular mechanism underlying the interaction between these two hormones are largely unexplored. In the present study, exogenous ABA treatment obviously promoted fruit ripening as well as...

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Bibliographic Details
Published in:PloS one 2016-04, Vol.11 (4), p.e0154072-e0154072
Main Authors: Mou, Wangshu, Li, Dongdong, Bu, Jianwen, Jiang, Yuanyuan, Khan, Zia Ullah, Luo, Zisheng, Mao, Linchun, Ying, Tiejin
Format: Article
Language:English
Subjects:
Abscisic acid
Abscisic Acid - metabolism
Biological effects
Biology and Life Sciences
Biosynthesis
Engineering
Enzymes
Ethylene
Ethylenes - metabolism
Food science
Fruits
Gene expression
Genetic aspects
Genomes
Genomics
Hormones
Laboratories
Lycopersicon esculentum - genetics
Lycopersicon esculentum - metabolism
Lycopersicon esculentum - physiology
Nordihydroguaiaretic acid
Physical Sciences
Physiological aspects
Physiology
Polyamines
Polymerase chain reaction
Regulators
Research and Analysis Methods
Ribonucleic acid
Ripening
Ripening (Botany)
RNA
RNA, Messenger - genetics
RNA, Plant - genetics
Sequence Analysis, RNA
Signal Transduction
Signaling
Studies
Tomatoes
Transcription factors
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Summary:ABA has been widely acknowledged to regulate ethylene biosynthesis and signaling during fruit ripening, but the molecular mechanism underlying the interaction between these two hormones are largely unexplored. In the present study, exogenous ABA treatment obviously promoted fruit ripening as well as ethylene emission, whereas NDGA (Nordihydroguaiaretic acid, an inhibitor of ABA biosynthesis) application showed the opposite biological effects. Combined RNA-seq with time-course RT-PCR analysis, our study not only helped to illustrate how ABA regulated itself at the transcription level, but also revealed that ABA can facilitate ethylene production and response probably by regulating some crucial genes such as LeACS4, LeACO1, GR and LeETR6. In addition, investigation on the fruits treated with 1-MCP immediately after ABA exposure revealed that ethylene might be essential for the induction of ABA biosynthesis and signaling at the onset of fruit ripening. Furthermore, some specific transcription factors (TFs) known as regulators of ethylene synthesis and sensibility (e.g. MADS-RIN, TAGL1, CNR and NOR) were also observed to be ABA responsive, which implied that ABA influenced ethylene action possibly through the regulation of these TFs expression. Our comprehensive physiological and molecular-level analysis shed light on the mechanism of cross-talk between ABA and ethylene during the process of tomato fruit ripening.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0154072