The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis

Ankyrin repeat and LEM-domain containing protein 1 (ANKLE1) is a GIY-YIG endonuclease with unknown functions, mainly expressed in mouse hematopoietic tissues. To test its potential role in hematopoiesis we generated Ankle1-deficient mice. Ankle1Δ/Δ mice are viable without any detectable phenotype in...

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Bibliographic Details
Published in:PloS one 2016-03, Vol.11 (3), p.e0152278-e0152278
Main Authors: Braun, Juliane, Meixner, Arabella, Brachner, Andreas, Foisner, Roland
Format: Article
Language:English
Subjects:
Animal tissues
Animals
Ankyrins
Biochemistry
Biology and Life Sciences
Bone marrow
Breast cancer
Cells (biology)
Deoxyribonucleic acid
DNA
DNA damage
DNA helicase
DNA repair
Embryo fibroblasts
Embryos
Endonuclease
Endonucleases - genetics
Endonucleases - physiology
Erythropoiesis
Fibroblasts
Flow Cytometry
Gene expression
Gene Knockdown Techniques
Genetic aspects
Genomes
Hematopoiesis
Hematopoiesis - physiology
Hematopoietic stem cells
Laboratories
Liver
Lymphocytes B
Lymphocytes T
Mammals
Medicine and Health Sciences
Mice
Mice, Inbred C57BL
Nucleases
Perutz, Max
Physiological aspects
Polymorphism, Single Nucleotide
Progenitor cells
Proteins
Repair
Research and Analysis Methods
RNA, Messenger - genetics
Rodents
Sister chromatid exchange
Spleen
Stem cells
Thymus
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Description
Summary:Ankyrin repeat and LEM-domain containing protein 1 (ANKLE1) is a GIY-YIG endonuclease with unknown functions, mainly expressed in mouse hematopoietic tissues. To test its potential role in hematopoiesis we generated Ankle1-deficient mice. Ankle1Δ/Δ mice are viable without any detectable phenotype in hematopoiesis. Neither hematopoietic progenitor cells, myeloid and lymphoid progenitors, nor B and T cell development in bone marrow, spleen and thymus, are affected in Ankle1Δ/Δ-mice. Similarly embryonic stress erythropoiesis in liver and adult erythropoiesis in bone marrow and spleen appear normal. To test whether ANKLE1, like the only other known GIY-YIG endonuclease in mammals, SLX1, may contribute to Holliday junction resolution during DNA repair, Ankle1-deficient cells were exposed to various DNA-damage inducing agents. However, lack of Ankle1 did not affect cell viability and, unlike depletion of Slx1, Ankle1-deficiency did not increase sister chromatid exchange in Bloom helicase-depleted cells. Altogether, we show that lack of Ankle1 does neither affect mouse hematopoiesis nor DNA damage repair in mouse embryonic fibroblasts, indicating a redundant or non-essential function of ANKLE1 in mouse.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0152278