Membrane Blebbing Is Required for Mesenchymal Precursor Migration

Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is...

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Bibliographic Details
Published in:PloS one 2016-03, Vol.11 (3), p.e0150004-e0150004
Main Authors: de Lucas, Beatriz, Bernal, Aurora, Pérez, Laura M, San Martín, Nuria, Gálvez, Beatriz G
Format: Article
Language:English
Subjects:
Actin
Actin Cytoskeleton - metabolism
Actins - metabolism
Animals
Biology and Life Sciences
Biomechanics
Cell adhesion & migration
Cell culture
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cell Membrane - drug effects
Cell Membrane - metabolism
Cell migration
Cell Movement - drug effects
Cell Movement - physiology
Contractility
Delivery contracts
Heterocyclic Compounds, 4 or More Rings - pharmacology
Immunofluorescence
Lamellipodia
Medicine and Health Sciences
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - drug effects
Mesenchymal Stem Cells - metabolism
Mesenchyme
Mice
Motility
Muscle proteins
Musculoskeletal system
Myosin
Physiological aspects
Political aspects
Polymerization
Precursors
Proteins
Pseudopodia
Pseudopodia - metabolism
Research and Analysis Methods
rhoA GTP-Binding Protein
RhoA protein
Stem cells
Stress analysis
Therapy
Tissues
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Summary:Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is thought to occur only through the formation of lamellipodia. More recently, contractility-driven bleb formation has emerged as an alternative mechanism of motility. Here we report that MPs derived from different tissues present spontaneously dynamic cytoplasmic projections in sub-confluent culture, which appear as a combination of lamellipodia with blebs in the leading edge. Upon initial seeding, however, only bleb structures could be observed. Immunofluorescence revealed the presence of pERM, RhoA and F-actin during the blebbing process. Results from migration assays in the presence of blebbistatin, a myosin II inhibitor, showed that bleb formation correlated with migratory capacity, suggesting a functional role for blebs in migration. Bleb formation might be a useful mechanism to improve cell migration in cellular therapy protocols.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0150004