MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis

This study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with sero...

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Bibliographic Details
Published in:PloS one 2015-12, Vol.10 (12), p.e0144534-e0144534
Main Authors: Ayo, Christiane Maria, Camargo, Ana Vitória da Silveira, Frederico, Fábio Batista, Siqueira, Rubens Camargo, Previato, Mariana, Murata, Fernando Henrique Antunes, Silveira-Carvalho, Aparecida Perpétuo, Barbosa, Amanda Pires, Brandão de Mattos, Cinara de Cássia, de Mattos, Luiz Carlos
Format: Article
Language:English
Subjects:
Adult
Aged
Alleles
Analysis
Brazil
Cytokines
Development and progression
Disease
Female
Gene Frequency
Genes
Genetic aspects
Genetic polymorphisms
Genotype
Genotyping
Haplotypes
Health aspects
Histocompatibility antigen HLA
Histocompatibility Antigens Class I - genetics
HLA antigens
HLA-B Antigens - genetics
HLA-C Antigens - genetics
Humans
Infections
Laboratories
Lesions
Linkage Disequilibrium
Lymphocytes
Major histocompatibility complex
Male
Middle Aged
Molecular biology
Ocular toxoplasmosis
Oligonucleotides
Pathogenesis
Patients
Physiological aspects
Polymerase chain reaction
Polymorphism
Polymorphism, Single Nucleotide
Population studies
Restriction fragment length polymorphism
Scars
Statistical analysis
Toxoplasma gondii
Toxoplasmosis
Toxoplasmosis, Ocular - diagnosis
Toxoplasmosis, Ocular - genetics
Tropical diseases
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Summary:This study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with serological diagnosis of toxoplasmosis. Participants were classified into two distinct groups after conducting fundoscopic exams according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis. The group of patients with scars/lesions was further subdivided into two groups according to the type of the ocular manifestation observed: primary (n = 120) or recurrent (n = 28). Genotyping of the MICA and HLA alleles was performed by the polymerase chain reaction-sequence specific oligonucleotide technique (PCR-SSO; One Lambda®) and the MICA-129 polymorphism (rs1051792) was identified by nested polymerase chain reaction (PCR-RFLP). Significant associations involving MICA polymorphisms were not found. Although the MICA*002~HLA-B*35 haplotype was associated with increased risk of developing ocular toxoplasmosis (P-value = 0.04; OR = 2.20; 95% CI = 1.05-4.60), and the MICA*008~HLA-C*07 haplotype was associated with protection against the development of manifestations of ocular toxoplasmosis (P-value = 0.009; OR: 0.44; 95% CI: 0.22-0.76), these associations were not statistically significant after adjusting for multiple comparisons. MICA polymorphisms do not appear to influence the development of ocular lesions in patients diagnosed with toxoplasmosis in this study population.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0144534