Glucocorticoid Metabolism in Hypertensive Disorders of Pregnancy: Analysis of Plasma and Urinary Cortisol and Cortisone

The aim of the study was to analyze the plasma and urinary cortisol (F) and cortisone (E) levels in normotensive and hypertensive pregnant women. The parameters known to reflect the function of 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) were calculated to verify the changes in glucocorticoid...

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Bibliographic Details
Published in:PloS one 2015-12, Vol.10 (12), p.e0144343-e0144343
Main Authors: Kosicka, Katarzyna, Siemiątkowska, Anna, Krzyścin, Mariola, Bręborowicz, Grzegorz H, Resztak, Matylda, Majchrzak-Celińska, Aleksandra, Chuchracki, Marek, Główka, Franciszek K
Format: Article
Language:English
Subjects:
11-beta-Hydroxysteroid Dehydrogenase Type 2 - metabolism
11β-Hydroxysteroid dehydrogenase
Abnormalities
Adolescent
Adult
Analysis
Babies
Birth
Birth weight
Care and treatment
Children
Complications and side effects
Control methods
Cortisol
Cortisone
Cortisone - blood
Cortisone - urine
Dehydrogenases
Disorders
Dosage and administration
Enzymes
Female
Gestation
Glucocorticoids
Glucocorticoids - blood
Glucocorticoids - urine
Growth hormone
Gynecology
Health aspects
Health risk assessment
High-performance liquid chromatography
Hormones
Humans
Hydrocortisone
Hydrocortisone - blood
Hydrocortisone - urine
Hypertension
Liquid chromatography
Mathematical analysis
Metabolism
Metabolites
Neonates
Newborn babies
Patients
Pharmacy
Physiological aspects
Plasma
Pre-eclampsia
Pre-Eclampsia - blood
Pre-Eclampsia - urine
Preeclampsia
Pregnancy
Pregnant women
Prenatal development
Retrospective Studies
Risk factors
Statistical analysis
Steroids (Organic compounds)
Urine
Womens health
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Summary:The aim of the study was to analyze the plasma and urinary cortisol (F) and cortisone (E) levels in normotensive and hypertensive pregnant women. The parameters known to reflect the function of 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) were calculated to verify the changes in glucocorticoid balance over the course of gestational hypertension (GH) and pre-eclampsia (PE). This retrospective case-control study included women in the third trimester of pregnancy, diagnosed with: GH (n = 29), PE (n = 26), or chronic hypertension (CH; n = 22). Normotensive women in their third trimester of pregnancy were also included (controls; n = 43). The plasma and urinary F and E levels were measured with the HPLC-FLD method. The 11β-HSD2 function was estimated by calculating the following ratios: plasma F/E and urinary free F to urinary free E (UFF/UFE). A statistical analysis was performed based on case-control structure. PE was characterized by lower plasma F levels (639.0 nmol/L), UFF/Cr levels (3.80 μg/mmol) and F/E ratio (3.46) compared with that of the controls (811.7 nmol/L, 6.28 μg/mmol and 5.19, respectively) with marked abnormalities observed in the changes of F/E and UFF/UFE ratios with advancing gestation. GH patients showed significant disparities in the urinary steroid profile with lower UFF/UFE ratio (0.330 vs. 0.401) compared with the normotensive controls and abnormal changes in the UFF/UFE throughout pregnancy. The observed tendency towards lower F/E and UFF/UFE ratios in PE and GH patients may reflect more intensive F metabolism over the course of those disorders. In the normal pregnancy group, the plasma F/E and UFF/UFE ratios tended to present inverse correlations with advancing gestation. This trend was much less marked in PE and GH patients, suggesting that the abnormalities in 11β-HSD2 functions progressed with the GA. The birth weights of neonates born from pre-eclamptic pregnancies were lower than those from uncomplicated pregnancies, although only when the babies were born prematurely. Children born at term to normotensive mothers or mothers suffering from PE had comparable birth weights.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0144343