Mast Cell Proteases 6 and 7 Stimulate Angiogenesis by Inducing Endothelial Cells to Release Angiogenic Factors

Mast cell proteases are thought to be involved with tumor progression and neo-vascularization. However, their exact role is still unclear. The present study was undertaken to further elucidate the function of specific subtypes of recombinant mouse mast cell proteases (rmMCP-6 and 7) in neo-vasculari...

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Bibliographic Details
Published in:PloS one 2015-12, Vol.10 (12), p.e0144081-e0144081
Main Authors: de Souza Junior, Devandir Antonio, Borges, Antonio Carlos, Santana, Ana Carolina, Oliver, Constance, Jamur, Maria Célia
Format: Article
Language:English
Subjects:
Angiogenesis
Angiogenesis Inducing Agents - pharmacology
Angiogenic Proteins - metabolism
Animals
Arthritis
Bone marrow
Cell culture
Cell growth
Cell Line
Cells, Cultured
Chick Embryo
Electron microscopy
Endothelial cells
Endothelial Cells - cytology
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Endothelium, Vascular - metabolism
Fluorescence
Fluorescence microscopy
Genetic aspects
Histamine
Incubation
Male
Mast cells
Mast Cells - cytology
Mast Cells - drug effects
Mast Cells - metabolism
Medical schools
Mice
Molecular biology
Neovascularization
Neovascularization, Pathologic - metabolism
Physiological aspects
Proteases
Proteins
Rodents
Scanning electron microscopy
Tryptases - pharmacology
Tumors
Vascular endothelial growth factor
Vascularization
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Summary:Mast cell proteases are thought to be involved with tumor progression and neo-vascularization. However, their exact role is still unclear. The present study was undertaken to further elucidate the function of specific subtypes of recombinant mouse mast cell proteases (rmMCP-6 and 7) in neo-vascularization. SVEC4-10 cells were cultured on Geltrex® with either rmMCP-6 or 7 and tube formation was analyzed by fluorescence microscopy and scanning electron microscopy. Additionally, the capacity of these proteases to induce the release of angiogenic factors and pro and anti-angiogenic proteins was analyzed. Both rmMCP-6 and 7 were able to stimulate tube formation. Scanning electron microscopy showed that incubation with the proteases induced SVEC4-10 cells to invade the gel matrix. However, the expression and activity of metalloproteases were not altered by incubation with the mast cell proteases. Furthermore, rmMCP-6 and rmMCP-7 were able to induce the differential release of angiogenic factors from the SVEC4-10 cells. rmMCP-7 was more efficient in stimulating tube formation and release of angiogenic factors than rmMCP-6. These results suggest that the subtypes of proteases released by mast cells may influence endothelial cells during in vivo neo-vascularization.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0144081