Silica Vesicle Nanovaccine Formulations Stimulate Long-Term Immune Responses to the Bovine Viral Diarrhoea Virus E2 Protein

Bovine Viral Diarrhoea Virus (BVDV) is one of the most serious pathogen, which causes tremendous economic loss to the cattle industry worldwide, meriting the development of improved subunit vaccines. Structural glycoprotein E2 is reported to be a major immunogenic determinant of BVDV virion. We have...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-12, Vol.10 (12), p.e0143507-e0143507
Main Authors: Mody, Karishma T, Mahony, Donna, Cavallaro, Antonino S, Zhang, Jun, Zhang, Bing, Mahony, Timothy J, Yu, Chengzhong, Mitter, Neena
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Agriculture
Animals
Antibody Formation
Antigens
Bioengineering
Blotting, Western
Bovine Virus Diarrhea-Mucosal Disease - immunology
Bovine Virus Diarrhea-Mucosal Disease - prevention & control
Care and treatment
Cattle
Cattle industry
Cold storage
Complications and side effects
Development and progression
Diarrhea
Diarrhea Virus 2, Bovine Viral - immunology
Drug Carriers - chemistry
E coli
E2 protein
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Female
Food
Formulations
Glycoprotein E2
Glycoproteins
Health aspects
Histopathology
Humans
Immune response
Immune system
Immunity
Immunity, Innate - immunology
Immunization
Immunoenzyme Techniques
Immunogenicity
Immunotherapy
Industrial development
Infections
Injections
Medical research
Mice
Mice, Inbred C57BL
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Nanoparticles - administration & dosage
Nanoparticles - chemistry
Nanotechnology
Organs
Patient outcomes
Proteins
Silica
Silicon dioxide
Silicon Dioxide - chemistry
Vaccines
Vesicles
Veterinary medicine
Viral Envelope Proteins - immunology
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
Virions
Virology
Viruses
Wall thickness
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bovine Viral Diarrhoea Virus (BVDV) is one of the most serious pathogen, which causes tremendous economic loss to the cattle industry worldwide, meriting the development of improved subunit vaccines. Structural glycoprotein E2 is reported to be a major immunogenic determinant of BVDV virion. We have developed a novel hollow silica vesicles (SV) based platform to administer BVDV-1 Escherichia coli-expressed optimised E2 (oE2) antigen as a nanovaccine formulation. The SV-140 vesicles (diameter 50 nm, wall thickness 6 nm, perforated by pores of entrance size 16 nm and total pore volume of 0.934 cm3 g(-1)) have proven to be ideal candidates to load oE2 antigen and generate immune response. The current study for the first time demonstrates the ability of freeze-dried (FD) as well as non-FD oE2/SV140 nanovaccine formulation to induce long-term balanced antibody and cell mediated memory responses for at least 6 months with a shortened dosing regimen of two doses in small animal model. The in vivo ability of oE2 (100 μg)/SV-140 (500 μg) and FD oE2 (100 μg)/SV-140 (500 μg) to induce long-term immunity was compared to immunisation with oE2 (100 μg) together with the conventional adjuvant Quil-A from the Quillaja saponira (10 μg) in mice. The oE2/SV-140 as well as the FD oE2/SV-140 nanovaccine generated oE2-specific antibody and cell mediated responses for up to six months post the final second immunisation. Significantly, the cell-mediated responses were consistently high in mice immunised with oE2/SV-140 (1,500 SFU/million cells) at the six-month time point. Histopathology studies showed no morphological changes at the site of injection or in the different organs harvested from the mice immunised with 500 μg SV-140 nanovaccine compared to the unimmunised control. The platform has the potential for developing single dose vaccines without the requirement of cold chain storage for veterinary and human applications.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0143507