Carbapenemase-Producing Klebsiella pneumoniae in Romania: A Six-Month Survey

This study presents the first characterization of carbapenem-non-susceptible Klebsiella pneumoniae isolates by means of a structured six-month survey performed in Romania as part of an Europe-wide investigation. Klebsiella pneumoniae clinical isolates from different anatomical sites were tested for...

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Bibliographic Details
Published in:PloS one 2015-11, Vol.10 (11), p.e0143214-e0143214
Main Authors: Lixandru, Brandusa Elena, Cotar, Ani Ioana, Straut, Monica, Usein, Codruta Romanita, Cristea, Dana, Ciontea, Simona, Tatu-Chitoiu, Dorina, Codita, Irina, Rafila, Alexandru, Nica, Maria, Buzea, Mariana, Baicus, Anda, Ghita, Mihaela Camelia, Nistor, Irina, Tuchiluş, Cristina, Indreas, Marina, Antohe, Felicia, Glasner, Corinna, Grundmann, Hajo, Jasir, Aftab, Damian, Maria
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Antimicrobial agents
Bacterial Proteins - biosynthesis
beta-Lactamases - biosynthesis
Carbapenemase
Care and treatment
Clinical isolates
Cross infection
Development and progression
Disease prevention
DNA fingerprinting
Electrophoresis, Gel, Pulsed-Field
Enterobacteriaceae
Epidemics
Genes
Genetic aspects
Genetic fingerprinting
Genomes
Genotypes
Health surveillance
Hospitals
Humans
Immunology
Infectious diseases
Klebsiella
Klebsiella pneumoniae
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - isolation & purification
Laboratories
Microbial Sensitivity Tests
Nosocomial infections
Patient outcomes
Phenotype
Pneumonia
Population
Production capacity
Public health
Risk factors
Romania
Studies
Surveys and Questionnaires
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Summary:This study presents the first characterization of carbapenem-non-susceptible Klebsiella pneumoniae isolates by means of a structured six-month survey performed in Romania as part of an Europe-wide investigation. Klebsiella pneumoniae clinical isolates from different anatomical sites were tested for antibiotic susceptibility by phenotypic methods and confirmed by PCR for the presence of four carbapenemase genes. Genome macrorestriction fingerprinting with XbaI was used to analyze the relatedness of carbapenemase-producing Klebsiella pneumoniae isolates collected from eight hospitals. Among 75 non-susceptible isolates, 65 were carbapenemase producers. The most frequently identified genotype was OXA-48 (n = 51 isolates), eight isolates were positive for blaNDM-1 gene, four had the blaKPC-2 gene, whereas two were positive for blaVIM-1. The analysis of PFGE profiles of OXA-48 and NDM-1 producing K. pneumoniae suggests inter-hospitals and regional transmission of epidemic clones. This study presents the first description of K. pneumoniae strains harbouring blaKPC-2 and blaVIM-1 genes in Romania. The results of this study highlight the urgent need for the strengthening of hospital infection control measures in Romania in order to curb the further spread of the antibiotic resistance.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0143214