Overexpression of Three Glucosinolate Biosynthesis Genes in Brassica napus Identifies Enhanced Resistance to Sclerotinia sclerotiorum and Botrytis cinerea

Sclerotinia sclerotiorum and Botrytis cinerea are notorious plant pathogenic fungi with an extensive host range including Brassica crops. Glucosinolates (GSLs) are an important group of secondary metabolites characteristic of the Brassicales order, whose degradation products are proving to be increa...

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Bibliographic Details
Published in:PloS one 2015-10, Vol.10 (10), p.e0140491-e0140491
Main Authors: Zhang, Yuanyuan, Huai, Dongxin, Yang, Qingyong, Cheng, Yan, Ma, Ming, Kliebenstein, Daniel J, Zhou, Yongming
Format: Article
Language:English
Subjects:
Aliphatic compounds
Arabidopsis
Arabidopsis Proteins - biosynthesis
Arabidopsis Proteins - genetics
Arabidopsis thaliana
Ascomycota - pathogenicity
Biodegradation
Biosynthesis
Botrytis
Botrytis - pathogenicity
Botrytis cinerea
Brassica
Brassica napus
Brassica napus - genetics
Brassica napus - growth & development
Brassica napus - microbiology
Brassica rapa
Brassicaceae
Cloning
Crops
Cytochrome P-450 Enzyme System - biosynthesis
Cytochrome P-450 Enzyme System - genetics
Degradation
Degradation products
Disease control
Disease Resistance - genetics
Fungi
Gene Expression Regulation, Plant
Genes
Genetic aspects
Genetic engineering
Genotype
Glucosinolates
Glucosinolates - metabolism
Glucosyltransferases - biosynthesis
Glucosyltransferases - genetics
Host range
Inoculation
Kinases
Laboratories
Leaves
Metabolites
Oxo-Acid-Lyases - biosynthesis
Oxo-Acid-Lyases - genetics
Pathogens
Physiology
Plant breeding
Plant Diseases - genetics
Plant Diseases - microbiology
Plant Leaves - genetics
Plant Leaves - microbiology
Plant metabolites
Plant protection
Plant sciences
Plants, Genetically Modified
Sclerotinia sclerotiorum
Secondary metabolites
Seeds
Transgenic plants
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Summary:Sclerotinia sclerotiorum and Botrytis cinerea are notorious plant pathogenic fungi with an extensive host range including Brassica crops. Glucosinolates (GSLs) are an important group of secondary metabolites characteristic of the Brassicales order, whose degradation products are proving to be increasingly important in plant protection. Enhancing the defense effect of GSL and their associated degradation products is an attractive strategy to strengthen the resistance of plants by transgenic approaches. We generated the lines of Brassica napus with three biosynthesis genes involved in GSL metabolic pathway (BnMAM1, BnCYP83A1 and BnUGT74B1), respectively. We then measured the foliar GSLs of each transgenic lines and inoculated them with S. sclerotiorum and B. cinerea. Compared with the wild type control, over-expressing BnUGT74B1 in B. napus increased the aliphatic and indolic GSL levels by 1.7 and 1.5 folds in leaves respectively; while over-expressing BnMAM1 or BnCYP83A1 resulted in an approximate 1.5-fold higher only in the aliphatic GSL level in leaves. The results of plant inoculation demonstrated that BnUGT74B1-overexpressing lines showed less severe disease symptoms and tissue damage compared with the wild type control, but BnMAM1 or BnCYP83A1-overexpressing lines showed no significant difference in comparison to the controls. These results suggest that the resistance to S. sclerotiorum and B. cinerea in B. napus could be enhanced through tailoring the GSL profiles by transgenic approaches or molecular breeding, which provides useful information to assist plant breeders to design improved breeding strategies.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0140491