Differential Metabolic Profiles during the Albescent Stages of 'Anji Baicha' (Camellia sinensis)

'Anji Baicha' is an albino tea cultivar with white shoots at low air temperature and green shoots at high air temperature in early spring. The metabolite contents in the shoots dynamically vary with the color changes and with shoot development. To investigate the metabolomic variation duri...

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Bibliographic Details
Published in:PloS one 2015-10, Vol.10 (10), p.e0139996-e0139996
Main Authors: Li, Chun-Fang, Yao, Ming-Zhe, Ma, Chun-Lei, Ma, Jian-Qiang, Jin, Ji-Qiang, Chen, Liang
Format: Article
Language:English
Subjects:
Acids
Agriculture
Air temperature
Albinism
Amino acids
Biology
Biosynthesis
Caffeine
Camellia sinensis
Camellia sinensis - growth & development
Camellia sinensis - metabolism
Carbohydrates
Carbon fixation
Chlorophyll
Chlorophyll - analysis
Chlorophyll - metabolism
Chromatography
Citrulline
Color
Cultivars
Developmental stages
Discriminant analysis
Epicatechin
Food
Fructose - analysis
Gas chromatography
Gas Chromatography-Mass Spectrometry
Gene expression
Genetic aspects
Glucose-1-phosphate
Glutamine
Glycine
Greening
Laboratories
Leaves
Mass spectrometry
Mass spectroscopy
Metabolic pathways
Metabolism
Metabolites
Metabolomics
Multivariate analysis
Pathways
Photosynthesis
Physiological aspects
Physiology
Plant Leaves - chemistry
Plant Leaves - growth & development
Plant Leaves - metabolism
Plant metabolism
Plant pigments
Principal components analysis
Proline
Scientific imaging
Serine
Shoots
Sugar
Tea
Tea (Plant)
Tryptophan
Valine
Zebrafish
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Summary:'Anji Baicha' is an albino tea cultivar with white shoots at low air temperature and green shoots at high air temperature in early spring. The metabolite contents in the shoots dynamically vary with the color changes and with shoot development. To investigate the metabolomic variation during the albescent and re-greening stages, gas chromatography-mass spectrometry combined with multivariate analysis were applied to analyze the metabolite profiles in the different color stages during the development of 'Anji Baicha' leaves. The metabolite profiles of three albescent stages, including the yellow-green stage, the early albescent stage, and the late albescent stage, as well as the re-greening stage were distinguished using principal component analysis, revealing that the distinct developmental stages were likely responsible for the observed metabolic differences. Furthermore, a group classification and pairwise discrimination was revealed among the three albescent stages and re-greening stage by partial least squares discriminant analysis. A total of 65 differential metabolites were identified with a variable influence on projection greater than 1. The main differential metabolic pathways of the albescent stages compared with the re-greening stage included carbon fixation in photosynthetic organisms and the phenylpropanoid and flavonoid biosynthesis pathways. Compared with the re-greening stage, the carbohydrate and amino acid metabolic pathways were disturbed during the albescent stages. During the albescent stages, the sugar (fructofuranose), sugar derivative (glucose-1-phosphate) and epicatechin concentrations decreased, whereas the amino acid (mainly glycine, serine, tryptophan, citrulline, glutamine, proline, and valine) concentrations increased. These results reveal the changes in metabolic profiling that occur during the color changes associated with the development of the albino tea plant leaves.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0139996