MicroRNA Expression in Myocardial Tissue and Plasma of Patients with End-Stage Heart Failure during LVAD Support: Comparison of Continuous and Pulsatile Devices

Pulsatile flow left ventricular assist devices (pf-LVADs) are being replaced by continuous flow LVADs (cf-LVADs) in patients with end-stage heart failure (HF). MicroRNAs (miRs) play an important role in the onset and progression of HF. Our aim was to analyze cardiac miR expression patterns associate...

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Published in:	PloS one 2015-10, Vol.10 (10), p.e0136404-e0136404
Main Authors:	Lok, Sjoukje I, de Jonge, Nicolaas, van Kuik, Joyce, van Geffen, Ankie J P, Huibers, Manon M H, van der Weide, Petra, Siera, Erica, Winkens, Bjorn, Doevendans, Pieter A, de Weger, Roel A, da Costa Martins, Paula A
Format:	Article
Language:	eng
Subjects:	Adult Analysis Angiogenesis Cardiology Cardiomyopathy Cardiovascular disease Care and treatment Continuous flow Demographics Female Gene expression Heart Heart diseases Heart failure Heart Failure - blood Heart Failure - metabolism Heart Failure - therapy Heart transplantation Heart-Assist Devices Humans Implantation Kinases Male MicroRNA MicroRNAs MicroRNAs - blood MicroRNAs - metabolism Middle Aged miRNA Myocardium - metabolism Pathology Patients Plasma Quality of life Ribonucleic acid Risk factors RNA Studies Transplantation Transplants & implants Variations Ventricle Ventricular assist devices
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Summary:	Pulsatile flow left ventricular assist devices (pf-LVADs) are being replaced by continuous flow LVADs (cf-LVADs) in patients with end-stage heart failure (HF). MicroRNAs (miRs) play an important role in the onset and progression of HF. Our aim was to analyze cardiac miR expression patterns associated with each type of device, to analyze differences in the regulation of the induced cardiac changes. Twenty-six miRs were selected (based on micro-array data and literature studies) and validated in myocardial tissue before and after pf- (n = 17) and cf-LVAD (n = 17) support. Of these, 5 miRs displayed a similar expression pattern among the devices (miR-129, miR-146a, miR-155, miR-221, miR-222), whereas others only changed significantly during pf-LVAD (miR-let-7i, miR-21, miR-378, miR-378) or cf-LVAD support (miR-137). In addition, 4 miRs were investigated in plasma of cf-LVAD supported patients (n = 18) and healthy controls (n = 10). Circulating miR-21 decreased at 1, 3, and 6 months after LVAD implantation. MiR-146a, miR-221 and miR-222 showed a fluctuating time pattern post-LVAD. Our data show a different miR expression pattern after LVAD support, suggesting that differentially expressed miRs are partially responsible for the cardiac morphological and functional changes observed after support. However, the miR expression patterns do not seem to significantly differ between pf- and cf-LVAD implying that most cardiac changes or clinical outcomes specific to each device do not relate to differences in miR expression levels.
ISSN:	1932-6203 1932-6203