Comparative Genotypes, Staphylococcal Cassette Chromosome mec (SCCmec) Genes and Antimicrobial Resistance amongst Staphylococcus epidermidis and Staphylococcus haemolyticus Isolates from Infections in Humans and Companion Animals

This study compares the characteristics of Staphylococcus epidermidis (SE) and Staphylococcus haemolyticus (SH) isolates from epidemiologically unrelated infections in humans (Hu) (28 SE-Hu; 8 SH-Hu) and companion animals (CpA) (12 SE-CpA; 13 SH-CpA). All isolates underwent antimicrobial susceptibil...

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Bibliographic Details
Published in:PloS one 2015-09, Vol.10 (9), p.e0138079-e0138079
Main Authors: McManus, Brenda A, Coleman, David C, Deasy, Emily C, Brennan, Gráinne I, O' Connell, Brian, Monecke, Stefan, Ehricht, Ralf, Leggett, Bernadette, Leonard, Nola, Shore, Anna C
Format: Article
Language:English
Subjects:
Accountants
Acid resistance
Alleles
Aminoglycosides
Animals
Anti-Bacterial Agents - therapeutic use
Antibacterial agents
Antiinfectives and antibacterials
Antimicrobial agents
Antimicrobial resistance
Bacterial Proteins - genetics
Cats
Chloramphenicol
Chromosomes
Ciprofloxacin
College campuses
Deoxyribonucleic acid
DNA
DNA fingerprinting
DNA microarrays
DNA sequencing
DNA, Bacterial - genetics
Dogs
Drug resistance
Drug Resistance, Multiple, Bacterial - genetics
Epidemiology
Fusidic acid
Genes
Genetic aspects
Genomics
Genotypes
Health aspects
Homology
Horses
Humans
Infection
Infections
Interspersed Repetitive Sequences - genetics
Laboratories
Methicillin
Methicillin Resistance - genetics
Microbial drug resistance
Microbial Sensitivity Tests
Molecular Sequence Data
Multiculturalism & pluralism
Multidrug resistance
Multilocus Sequence Typing
Nosocomial infections
Nucleotide sequence
Oligonucleotide Array Sequence Analysis
Pets - microbiology
Population
Rifampin
Staphylococcal infections
Staphylococcal Infections - drug therapy
Staphylococcal Infections - microbiology
Staphylococcus
Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus epidermidis - drug effects
Staphylococcus epidermidis - genetics
Staphylococcus epidermidis - isolation & purification
Staphylococcus haemolyticus - drug effects
Staphylococcus haemolyticus - genetics
Staphylococcus haemolyticus - isolation & purification
Staphylococcus infections
Studies
Trimethoprim
Veterinary colleges
Veterinary medicine
Working groups
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Summary:This study compares the characteristics of Staphylococcus epidermidis (SE) and Staphylococcus haemolyticus (SH) isolates from epidemiologically unrelated infections in humans (Hu) (28 SE-Hu; 8 SH-Hu) and companion animals (CpA) (12 SE-CpA; 13 SH-CpA). All isolates underwent antimicrobial susceptibility testing, multilocus sequence typing and DNA microarray profiling to detect antimicrobial resistance and SCCmec-associated genes. All methicillin-resistant (MR) isolates (33/40 SE, 20/21 SH) underwent dru and mecA allele typing. Isolates were predominantly assigned to sequence types (STs) within a single clonal complex (CC2, SE, 84.8%; CC1, SH, 95.2%). SCCmec IV predominated among MRSE with ST2-MRSE-IVc common to both Hu (40.9%) and CpA (54.5%). Identical mecA alleles and nontypeable dru types (dts) were identified in one ST2-MRSE-IVc Hu and CpA isolate, however, all mecA alleles and 2/4 dts detected among 18 ST2-MRSE-IVc isolates were closely related, sharing >96.5% DNA sequence homology. Although only one ST-SCCmec type combination (ST1 with a non-typeable [NT] SCCmec NT9 [class C mec and ccrB4]) was common to four MRSH-Hu and one MRSH-CpA, all MRSH isolates were closely related based on similar STs, SCCmec genes (V/VT or components thereof), mecA alleles and dts. Overall, 39.6% of MR isolates harbored NT SCCmec elements, and ACME was more common amongst MRSE and CpA isolates. Multidrug resistance (MDR) was detected among 96.7% of isolates but they differed in the prevalence of specific macrolide, aminoglycoside and trimethoprim resistance genes amongst SE and SH isolates. Ciprofloxacin, rifampicin, chloramphenicol [fexA, cat-pC221], tetracycline [tet(K)], aminoglycosides [aadD, aphA3] and fusidic acid [fusB] resistance was significantly more common amongst CpA isolates. SE and SH isolates causing infections in Hu and CpA hosts belong predominantly to STs within a single lineage, harboring similar but variable SCCmec genes, mecA alleles and dts. Host and staphylococcal species-specific characteristics were identified in relation to antimicrobial resistance genes and phenotypes, SCCmec and ACME.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0138079