Large-Scale Phenomics Identifies Primary and Fine-Tuning Roles for CRKs in Responses Related to Oxidative Stress

Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains of unknown function 26 (DUF26) in their ectodomain. The CRKs form one of the largest groups of receptor-like protein kinases in plants, but their biological functions have so far remain...

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Published in:PLoS genetics 2015-07, Vol.11 (7), p.e1005373-e1005373
Main Authors: Bourdais, Gildas, Burdiak, Paweł, Gauthier, Adrien, Nitsch, Lisette, Salojärvi, Jarkko, Rayapuram, Channabasavangowda, Idänheimo, Niina, Hunter, Kerri, Kimura, Sachie, Merilo, Ebe, Vaattovaara, Aleksia, Oracz, Krystyna, Kaufholdt, David, Pallon, Andres, Anggoro, Damar Tri, Glów, Dawid, Lowe, Jennifer, Zhou, Ji, Mohammadi, Omid, Puukko, Tuomas, Albert, Andreas, Lang, Hans, Ernst, Dieter, Kollist, Hannes, Brosché, Mikael, Durner, Jörg, Borst, Jan Willem, Collinge, David B, Karpiński, Stanisław, Lyngkjær, Michael F, Robatzek, Silke, Wrzaczek, Michael, Kangasjärvi, Jaakko
Format: Article
Language:English
Subjects:
Adaptation
Adaptation, Physiological - genetics
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis - immunology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
arabidopsis-thaliana
Ascomycota - immunology
Biochemistry, Molecular Biology
cell-death
Charitable foundations
Consortia
Councils
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Experiments
flagellin perception
Funding
Gene Expression Regulation, Plant
Genomes
Kinases
Laboratories
Life Sciences
light acclimation
multiple sequence alignment
Oxidative stress
Oxidative Stress - immunology
Phytopathology and phytopharmacy
Plant Diseases - immunology
Plant Diseases - microbiology
Protein Serine-Threonine Kinases - genetics
Protein Serine-Threonine Kinases - metabolism
protein-kinase
Proteins
Pseudomonas syringae - immunology
pseudomonas-syringae
Reactive Oxygen Species - metabolism
receptor-like kinase
Science
Signal transduction
Signal Transduction - genetics
stomatal immunity
transcriptional regulation
Vegetal Biology
Xanthine Oxidase - metabolism
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Summary:Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains of unknown function 26 (DUF26) in their ectodomain. The CRKs form one of the largest groups of receptor-like protein kinases in plants, but their biological functions have so far remained largely uncharacterized. We conducted a large-scale phenotyping approach of a nearly complete crk T-DNA insertion line collection showing that CRKs control important aspects of plant development and stress adaptation in response to biotic and abiotic stimuli in a non-redundant fashion. In particular, the analysis of reactive oxygen species (ROS)-related stress responses, such as regulation of the stomatal aperture, suggests that CRKs participate in ROS/redox signalling and sensing. CRKs play general and fine-tuning roles in the regulation of stomatal closure induced by microbial and abiotic cues. Despite their great number and high similarity, large-scale phenotyping identified specific functions in diverse processes for many CRKs and indicated that CRK2 and CRK5 play predominant roles in growth regulation and stress adaptation, respectively. As a whole, the CRKs contribute to specificity in ROS signalling. Individual CRKs control distinct responses in an antagonistic fashion suggesting future potential for using CRKs in genetic approaches to improve plant performance and stress tolerance.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1005373