Magnetic Clustering Effect during the Association of Biofunctionalized Magnetic Nanoparticles with Biomarkers

We report herein an investigation into dynamic magnetic clustering that occurs during immunoassays as biofunctionalized magnetic nanoparticles (BMNs) become associated with biotargets. We measure the dynamic effective relaxation time τeff(t) and use scanning electron microscopy (SEM) and transmissio...

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Bibliographic Details
Published in:PloS one 2015-08, Vol.10 (8), p.e0135290-e0135290
Main Authors: Chen, Kuen-Lin, Chen, Jean-Hong, Liao, Su-Hsien, Chieh, Jen-Je, Horng, Herng-Er, Wang, Li-Min, Yang, Hong-Chang
Format: Article
Language:English
Subjects:
Bioindicators
Biomarkers
Biomarkers - chemistry
Biomarkers - metabolism
Biomolecules
C-reactive protein
C-Reactive Protein - chemistry
C-Reactive Protein - metabolism
Clustering
Electron microscopy
Ferrosoferric Oxide - chemistry
Humans
Immunoassay
Immunoassays
Iron oxides
Magnetite Nanoparticles - chemistry
Measurement techniques
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Nanoparticles
Particle Size
Physics
Proteins
Relaxation time
Scanning electron microscopy
Transmission electron microscopy
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Summary:We report herein an investigation into dynamic magnetic clustering that occurs during immunoassays as biofunctionalized magnetic nanoparticles (BMNs) become associated with biotargets. We measure the dynamic effective relaxation time τeff(t) and use scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to investigate the C-reactive protein (CRP) as it associates with the BMN Fe3O4-antiCRP to form the magnetic cluster Fe3O4-antiCRP-CRP. The results indicate that τeff(t) increases with increasing association time. In addition, the ration Δτeff/τ0 as a function of CRP concentration follows a characteristic logistic function, which provides a basis for estimating the quantity of biomolecules with a detection sensitivity close to 0.1 ppm. After the association, SEM and TEM images show that CRP and Fe3O4-antiCRP conjugate to form Fe3O4-antiCRP-CRP clusters hundreds of nanometers in size. The SEM and TEM images provide direct evidence of the formation of magnetic clustering.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0135290