Expression of OsCAS (Calcium-Sensing Receptor) in an Arabidopsis Mutant Increases Drought Tolerance

The calcium-sensing receptor (CaS), which is localized in the chloroplasts, is a crucial regulator of extracellular calcium-induced stomatal closure in Arabidopsis. It has homologs in Oryza sativa and other plants. These sequences all have a rhodanese-like protein domain, which has been demonstrated...

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Bibliographic Details
Published in:PloS one 2015-06, Vol.10 (6), p.e0131272-e0131272
Main Authors: Zhao, Xin, Xu, Mengmeng, Wei, Rongrong, Liu, Yang
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - genetics
Arabidopsis - physiology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - physiology
Arabidopsis thaliana
Barley
Bioindicators
Biology
Biomarkers
Calcium
Calcium (extracellular)
Calcium - analysis
Calcium ions
Calcium-Binding Proteins - genetics
Calcium-Binding Proteins - physiology
Chlorophyll
Chloroplasts
Cloning
Cytosol - chemistry
Dehydration - genetics
Dehydration - physiopathology
Deoxyribonucleic acid
Detection
DNA
Drought
Drought damage
Drought resistance
Droughts
Electron transport
Energy dissipation
Flowers & plants
Fluorescence
Gene Knockout Techniques
Genetic engineering
Genetic research
Genetically modified plants
HEK293 Cells
Homology
Humans
Humidity
Life sciences
Light
Malondialdehyde
Membrane permeability
Mutation
Oryza sativa
Osmoprotectants
Parameters
Permeability
Photobiology
Photochemistry
Photosynthesis
Photosystem II
Physiological aspects
Physiology
Plant Stomata - physiology
Plant tolerance
Plants (botany)
Plants, Genetically Modified
Proline
Proteins
Receptors, Calcium-Sensing - genetics
Receptors, Calcium-Sensing - physiology
Rhodanese
Seeds
Stomata
Stress
Stresses
Transgenic plants
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Summary:The calcium-sensing receptor (CaS), which is localized in the chloroplasts, is a crucial regulator of extracellular calcium-induced stomatal closure in Arabidopsis. It has homologs in Oryza sativa and other plants. These sequences all have a rhodanese-like protein domain, which has been demonstrated to be associated with specific stress conditions. In this study, we cloned the Oryza sativa calcium-sensing receptor gene (OsCAS) and demonstrated that OsCAS could sense an increase of extracellular Ca2+ concentration and mediate an increase in cytosolic Ca2+ concentration. The OsCAS gene was transformed into an Arabidopsis CaS knockout mutant (Salk) and overexpressed in the transgenic plants. OsCAS promoted stomatal closure. We screened homozygous transgenic Arabidopsis plants and determined physiological indices such as the oxidative damage biomarker malondialdehyde (MDA), relative membrane permeability (RMP), proline content, and chlorophyll fluorescence parameters, after 21 days of drought treatment. Our results revealed lower RMP and MDA contents and a higher Proline content in transgenic Arabidopsis plants after drought stress, whereas the opposite was observed in Salk plants. With respect to chlorophyll fluorescence, the electron transport rate and effective PSII quantum yield decreased in all lines under drought stress; however, in the transgenic plants these two parameters changed fewer and were higher than those in wild-type and Salk plants. The quantum yield of regulated energy dissipation and nonregulated energy dissipation in PSII were higher in Salk plants, whereas these values were lower in the transgenic plants than in the wild type under drought stress. The above results suggest that the transgenic plants showed better resistance to drought stress by decreasing damage to the cell membrane, increasing the amount of osmoprotectants, and maintaining a relatively high photosynthetic capacity. In conclusion, OsCAS is an extracellular calcium-sensing receptor that helps to compensate for the absence of CaS in Arabidopsis and increases the drought stress tolerance of transgenic plants.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0131272