Combinatorial control of light induced chromatin remodeling and gene activation in Neurospora

Light is an important environmental cue that affects physiology and development of Neurospora crassa. The light-sensing transcription factor (TF) WCC, which consists of the GATA-family TFs WC1 and WC2, is required for light-dependent transcription. SUB1, another GATA-family TF, is not a photorecepto...

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Bibliographic Details
Published in:PLoS genetics 2015-03, Vol.11 (3), p.e1005105-e1005105
Main Authors: Sancar, Cigdem, Ha, Nati, Yilmaz, Rüstem, Tesorero, Rafael, Fisher, Tamas, Brunner, Michael, Sancar, Gencer
Format: Article
Language:English
Subjects:
Binding sites
Chromatin
Chromatin Assembly and Disassembly - genetics
Chromatin Assembly and Disassembly - radiation effects
Circadian rhythm
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
Fungal Proteins - biosynthesis
Fungal Proteins - genetics
Gene expression
Gene Expression Regulation, Fungal - genetics
Gene Expression Regulation, Fungal - radiation effects
Genetic aspects
Identification and classification
Light
Neurospora
Neurospora crassa - genetics
Neurospora crassa - radiation effects
Photoreceptors
Physiological aspects
Physiology
Signal transduction
Transcription factors
Transcription Factors - biosynthesis
Transcription Factors - genetics
Transcriptional Activation - genetics
Transcriptional Activation - radiation effects
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Summary:Light is an important environmental cue that affects physiology and development of Neurospora crassa. The light-sensing transcription factor (TF) WCC, which consists of the GATA-family TFs WC1 and WC2, is required for light-dependent transcription. SUB1, another GATA-family TF, is not a photoreceptor but has also been implicated in light-inducible gene expression. To assess regulation and organization of the network of light-inducible genes, we analyzed the roles of WCC and SUB1 in light-induced transcription and nucleosome remodeling. We show that SUB1 co-regulates a fraction of light-inducible genes together with the WCC. WCC induces nucleosome eviction at its binding sites. Chromatin remodeling is facilitated by SUB1 but SUB1 cannot activate light-inducible genes in the absence of WCC. We identified FF7, a TF with a putative O-acetyl transferase domain, as an interaction partner of SUB1 and show their cooperation in regulation of a fraction of light-inducible and a much larger number of non light-inducible genes. Our data suggest that WCC acts as a general switch for light-induced chromatin remodeling and gene expression. SUB1 and FF7 synergistically determine the extent of light-induction of target genes in common with WCC but have in addition a role in transcription regulation beyond light-induced gene expression.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1005105