Serology of paracoccidioidomycosis due to Paracoccidioides lutzii

Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply t...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2014-07, Vol.8 (7), p.e2986-e2986
Main Authors: Gegembauer, Gregory, Araujo, Leticia Mendes, Pereira, Edy Firmina, Rodrigues, Anderson Messias, Paniago, Anamaria Mello Miranda, Hahn, Rosane Christine, de Camargo, Zoilo Pires
Format: Article
Language:English
Subjects:
Adult
Aged
Antigens
Antigens, Fungal - immunology
Antigens, Fungal - isolation & purification
Biology and Life Sciences
Blotting, Western
Brazil
Chronic illnesses
Diagnosis
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunodiffusion
Laboratories
Male
Medical research
Medicine, Experimental
Middle Aged
Molecular weight
Mortality
Paracoccidioides
Paracoccidioides - immunology
Paracoccidioides - isolation & purification
Paracoccidioides brasiliensis
Paracoccidioidomycosis
Paracoccidioidomycosis - diagnosis
Paracoccidioidomycosis - immunology
Paracoccidioidomycosis - microbiology
Patients
Research and Analysis Methods
Serology
Young Adult
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Summary:Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. In western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0002986