The Neisseria meningitidis ADP-Ribosyltransferase NarE Enters Human Epithelial Cells and Disrupts Epithelial Monolayer Integrity

Many pathogenic bacteria utilize ADP-ribosylating toxins to modify and impair essential functions of eukaryotic cells. It has been previously reported that Neisseria meningitidis possesses an ADP-ribosyltransferase enzyme, NarE, retaining the capacity to hydrolyse NAD and to transfer ADP-ribose moie...

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Bibliographic Details
Published in:PloS one 2015-05, Vol.10 (5), p.e0127614-e0127614
Main Authors: Valeri, Maria, Zurli, Vanessa, Ayala, Inmaculada, Colanzi, Antonino, Lapazio, Lucia, Corda, Daniela, Soriani, Marco, Pizza, Mariagrazia, Rossi Paccani, Silvia
Format: Article
Language:English
Subjects:
Actins - metabolism
Adenosine diphosphate
ADP Ribose Transferases - metabolism
Analysis
Animals
Apoptosis
Arginine
Bacillus cereus
Bacteria
Biochemistry
Biological activity
Cholera
Clostridium
Councils
Cytoplasm
E coli
Endocytosis
Enzymes
Epithelial cells
Epithelial Cells - metabolism
Epithelial Cells - pathology
Epithelial Cells - virology
HeLa Cells
Humans
Immunoglobulins
Infections
Influence
Integrity
Internalization
Intracellular Space - metabolism
Laboratory animals
Mice
Monolayers
Monomolecular films
Monosaccharides
NAD
Neisseria meningitidis
Neisseria meningitidis - metabolism
Pathogenesis
Physiological aspects
Protein Binding
Protein Transport
Proteins
Ribose
Salmonella
Signal transduction
Toxins
Vaccines
Whooping cough
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Summary:Many pathogenic bacteria utilize ADP-ribosylating toxins to modify and impair essential functions of eukaryotic cells. It has been previously reported that Neisseria meningitidis possesses an ADP-ribosyltransferase enzyme, NarE, retaining the capacity to hydrolyse NAD and to transfer ADP-ribose moiety to arginine residues in target acceptor proteins. Here we show that upon internalization into human epithelial cells, NarE gains access to the cytoplasm and, through its ADP-ribosylating activity, targets host cell proteins. Notably, we observed that these events trigger the disruption of the epithelial monolayer integrity and the activation of the apoptotic pathway. Overall, our findings provide, for the first time, evidence for a biological activity of NarE on host cells, suggesting its possible involvement in Neisseria pathogenesis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0127614