Loading…
Genomic Characterizations of Six Pigeon Paramyxovirus Type 1 Viruses Isolated from Live Bird Markets in China during 2011 to 2013
The genomes of six pigeon paramyxovirus type 1 (PPMV-1) isolated from symptomless pigeons in live poultry markets during the national active surveillance from 2011 to 2013 were sequenced and analyzed in this study. The complete genome lengths of all isolates were 15,192 nucleotides with the gene ord...
Saved in:
| Published in: | PloS one 2015-04, Vol.10 (4), p.e0124261-e0124261 |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c692t-50b509e7f857c97cb580dbc890677d846e1d549d392e79ffa216839b4eebd1793 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c692t-50b509e7f857c97cb580dbc890677d846e1d549d392e79ffa216839b4eebd1793 |
| container_end_page | e0124261 |
| container_issue | 4 |
| container_start_page | e0124261 |
| container_title | PloS one |
| container_volume | 10 |
| creator | Wang, Jingjing Liu, Hualei Liu, Wei Zheng, Dongxia Zhao, Yunling Li, Yin Wang, Yingli Ge, Shengqiang Lv, Yan Zuo, Yuanyuan Yu, Songmei Wang, Zhiliang |
| description | The genomes of six pigeon paramyxovirus type 1 (PPMV-1) isolated from symptomless pigeons in live poultry markets during the national active surveillance from 2011 to 2013 were sequenced and analyzed in this study. The complete genome lengths of all isolates were 15,192 nucleotides with the gene order of 3'-NP-P-M-F-HN-L-5'. All isolates had the same motif of 112RRQKRF117 at the cleavage site of the fusion protein, which was typical of velogenic Newcastle disease virus (NDV). Several mutations were identified in the functional domains of F and HN proteins, including fusion peptide, heptad repeat region, transmembrane domains and neutralizing epitopes. Phylogenetic analysis based on sequences of complete genomes and six genes revealed that all isolates belonged to genotype VI in class II, but at least 2 sub-genotypes were identified. Most isolates were placed into sub-genotype VIb with the exception of pi/GX/1015/13, which was classified in sub-genotype VIa. The obvious antigenic difference between PPMV-1 isolates and La Sota strain was found based on the R-value calculated by cross hemagglutination inhibition (HI) assay. These results provided the evidence that PPMV-1 could be detected from healthy pigeons, and our study may be useful in designing vaccines used in pigeon, and developing molecular diagnostic tools to monitor and prevent future PPMV-1 outbreaks. |
| doi_str_mv | 10.1371/journal.pone.0124261 |
| format | article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1677376606</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A419035986</galeid><doaj_id>oai_doaj_org_article_9b5c7fc44ea8467c96417b311476c553</doaj_id><sourcerecordid>A419035986</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-50b509e7f857c97cb580dbc890677d846e1d549d392e79ffa216839b4eebd1793</originalsourceid><addsrcrecordid>eNqNk01v1DAQhiMEoqXwDxBYQkJw2MWOv-ILUllBWamoFS29Wo4z2XVJ4sVOVltu_HOcdlt1UQ8oByf2874znsxk2UuCp4RK8uHSD6EzzXTlO5hikrNckEfZPlE0n4gc08f33veyZzFeYsxpIcTTbC_nKi8wl_vZnyPofOssmi1NMLaH4H6b3vkuIl-jM7dBp24BvkOn6bi92vi1C0NE51crQARdjB8Q0Tz6xvRQoTr4Fh27NaBPLlTomwk_oY_IdcnfdQZVQ3DdAuWYENT7caXPsye1aSK82K4H2Y8vn89nXyfHJ0fz2eHxxAqV9xOOS44VyLrg0ippS17gqrSFwkLKqmACSMWZqqjKQaq6NjkRBVUlAygrIhU9yF7f-K4aH_W2elGTJKdSCCwSMb8hKm8u9Sq41oQr7Y3T1xs-LLQJvbMNaFVyK2vLGJgUOiUkGJElJYRJYTmnyevjNtpQtlBZ6Ppgmh3T3ZPOLfXCrzVjhKd0ksG7rUHwvwaIvW5dtNA0pgM_jHkXmEhZUJbQN_-gD99uSy1MuoDrap_i2tFUHzKiMOWqGKnpA1R6Kkhdklqtdml_R_B-R5CYHjb9wgwx6vnZ9_9nTy522bf32CWYpl-mLhuue3MXZDegDT7GAPVdkQnW46TcVkOPk6K3k5Jkr-7_oDvR7WjQvyT7DAM</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1677376606</pqid></control><display><type>article</type><title>Genomic Characterizations of Six Pigeon Paramyxovirus Type 1 Viruses Isolated from Live Bird Markets in China during 2011 to 2013</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Wang, Jingjing ; Liu, Hualei ; Liu, Wei ; Zheng, Dongxia ; Zhao, Yunling ; Li, Yin ; Wang, Yingli ; Ge, Shengqiang ; Lv, Yan ; Zuo, Yuanyuan ; Yu, Songmei ; Wang, Zhiliang</creator><contributor>Samal, Siba K</contributor><creatorcontrib>Wang, Jingjing ; Liu, Hualei ; Liu, Wei ; Zheng, Dongxia ; Zhao, Yunling ; Li, Yin ; Wang, Yingli ; Ge, Shengqiang ; Lv, Yan ; Zuo, Yuanyuan ; Yu, Songmei ; Wang, Zhiliang ; Samal, Siba K</creatorcontrib><description>The genomes of six pigeon paramyxovirus type 1 (PPMV-1) isolated from symptomless pigeons in live poultry markets during the national active surveillance from 2011 to 2013 were sequenced and analyzed in this study. The complete genome lengths of all isolates were 15,192 nucleotides with the gene order of 3'-NP-P-M-F-HN-L-5'. All isolates had the same motif of 112RRQKRF117 at the cleavage site of the fusion protein, which was typical of velogenic Newcastle disease virus (NDV). Several mutations were identified in the functional domains of F and HN proteins, including fusion peptide, heptad repeat region, transmembrane domains and neutralizing epitopes. Phylogenetic analysis based on sequences of complete genomes and six genes revealed that all isolates belonged to genotype VI in class II, but at least 2 sub-genotypes were identified. Most isolates were placed into sub-genotype VIb with the exception of pi/GX/1015/13, which was classified in sub-genotype VIa. The obvious antigenic difference between PPMV-1 isolates and La Sota strain was found based on the R-value calculated by cross hemagglutination inhibition (HI) assay. These results provided the evidence that PPMV-1 could be detected from healthy pigeons, and our study may be useful in designing vaccines used in pigeon, and developing molecular diagnostic tools to monitor and prevent future PPMV-1 outbreaks.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0124261</identifier><identifier>PMID: 25928057</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal vaccines ; Animals ; Antigenic determinants ; Antigens ; Birds ; Birds - virology ; Chemical properties ; Cladistic analysis ; Diagnostic software ; Diagnostic systems ; Epitopes ; Fusion protein ; Gene order ; Gene sequencing ; Genome, Viral - genetics ; Genomes ; Genomics ; Genotypes ; Hemagglutination inhibition ; Markets ; Mutation ; Newcastle disease ; Newcastle disease virus - classification ; Newcastle disease virus - genetics ; Nucleotides ; Outbreaks ; Phylogeny ; Pigeons ; Poultry ; Proteins ; Transmembrane domains ; Vaccines ; Viruses</subject><ispartof>PloS one, 2015-04, Vol.10 (4), p.e0124261-e0124261</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Wang et al 2015 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-50b509e7f857c97cb580dbc890677d846e1d549d392e79ffa216839b4eebd1793</citedby><cites>FETCH-LOGICAL-c692t-50b509e7f857c97cb580dbc890677d846e1d549d392e79ffa216839b4eebd1793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1677376606/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1677376606?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,25783,27957,27958,37047,37048,44625,53827,53829,75483</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25928057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Samal, Siba K</contributor><creatorcontrib>Wang, Jingjing</creatorcontrib><creatorcontrib>Liu, Hualei</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Zheng, Dongxia</creatorcontrib><creatorcontrib>Zhao, Yunling</creatorcontrib><creatorcontrib>Li, Yin</creatorcontrib><creatorcontrib>Wang, Yingli</creatorcontrib><creatorcontrib>Ge, Shengqiang</creatorcontrib><creatorcontrib>Lv, Yan</creatorcontrib><creatorcontrib>Zuo, Yuanyuan</creatorcontrib><creatorcontrib>Yu, Songmei</creatorcontrib><creatorcontrib>Wang, Zhiliang</creatorcontrib><title>Genomic Characterizations of Six Pigeon Paramyxovirus Type 1 Viruses Isolated from Live Bird Markets in China during 2011 to 2013</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The genomes of six pigeon paramyxovirus type 1 (PPMV-1) isolated from symptomless pigeons in live poultry markets during the national active surveillance from 2011 to 2013 were sequenced and analyzed in this study. The complete genome lengths of all isolates were 15,192 nucleotides with the gene order of 3'-NP-P-M-F-HN-L-5'. All isolates had the same motif of 112RRQKRF117 at the cleavage site of the fusion protein, which was typical of velogenic Newcastle disease virus (NDV). Several mutations were identified in the functional domains of F and HN proteins, including fusion peptide, heptad repeat region, transmembrane domains and neutralizing epitopes. Phylogenetic analysis based on sequences of complete genomes and six genes revealed that all isolates belonged to genotype VI in class II, but at least 2 sub-genotypes were identified. Most isolates were placed into sub-genotype VIb with the exception of pi/GX/1015/13, which was classified in sub-genotype VIa. The obvious antigenic difference between PPMV-1 isolates and La Sota strain was found based on the R-value calculated by cross hemagglutination inhibition (HI) assay. These results provided the evidence that PPMV-1 could be detected from healthy pigeons, and our study may be useful in designing vaccines used in pigeon, and developing molecular diagnostic tools to monitor and prevent future PPMV-1 outbreaks.</description><subject>Animal vaccines</subject><subject>Animals</subject><subject>Antigenic determinants</subject><subject>Antigens</subject><subject>Birds</subject><subject>Birds - virology</subject><subject>Chemical properties</subject><subject>Cladistic analysis</subject><subject>Diagnostic software</subject><subject>Diagnostic systems</subject><subject>Epitopes</subject><subject>Fusion protein</subject><subject>Gene order</subject><subject>Gene sequencing</subject><subject>Genome, Viral - genetics</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Genotypes</subject><subject>Hemagglutination inhibition</subject><subject>Markets</subject><subject>Mutation</subject><subject>Newcastle disease</subject><subject>Newcastle disease virus - classification</subject><subject>Newcastle disease virus - genetics</subject><subject>Nucleotides</subject><subject>Outbreaks</subject><subject>Phylogeny</subject><subject>Pigeons</subject><subject>Poultry</subject><subject>Proteins</subject><subject>Transmembrane domains</subject><subject>Vaccines</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBYQkJw2MWOv-ILUllBWamoFS29Wo4z2XVJ4sVOVltu_HOcdlt1UQ8oByf2874znsxk2UuCp4RK8uHSD6EzzXTlO5hikrNckEfZPlE0n4gc08f33veyZzFeYsxpIcTTbC_nKi8wl_vZnyPofOssmi1NMLaH4H6b3vkuIl-jM7dBp24BvkOn6bi92vi1C0NE51crQARdjB8Q0Tz6xvRQoTr4Fh27NaBPLlTomwk_oY_IdcnfdQZVQ3DdAuWYENT7caXPsye1aSK82K4H2Y8vn89nXyfHJ0fz2eHxxAqV9xOOS44VyLrg0ippS17gqrSFwkLKqmACSMWZqqjKQaq6NjkRBVUlAygrIhU9yF7f-K4aH_W2elGTJKdSCCwSMb8hKm8u9Sq41oQr7Y3T1xs-LLQJvbMNaFVyK2vLGJgUOiUkGJElJYRJYTmnyevjNtpQtlBZ6Ppgmh3T3ZPOLfXCrzVjhKd0ksG7rUHwvwaIvW5dtNA0pgM_jHkXmEhZUJbQN_-gD99uSy1MuoDrap_i2tFUHzKiMOWqGKnpA1R6Kkhdklqtdml_R_B-R5CYHjb9wgwx6vnZ9_9nTy522bf32CWYpl-mLhuue3MXZDegDT7GAPVdkQnW46TcVkOPk6K3k5Jkr-7_oDvR7WjQvyT7DAM</recordid><startdate>20150430</startdate><enddate>20150430</enddate><creator>Wang, Jingjing</creator><creator>Liu, Hualei</creator><creator>Liu, Wei</creator><creator>Zheng, Dongxia</creator><creator>Zhao, Yunling</creator><creator>Li, Yin</creator><creator>Wang, Yingli</creator><creator>Ge, Shengqiang</creator><creator>Lv, Yan</creator><creator>Zuo, Yuanyuan</creator><creator>Yu, Songmei</creator><creator>Wang, Zhiliang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150430</creationdate><title>Genomic Characterizations of Six Pigeon Paramyxovirus Type 1 Viruses Isolated from Live Bird Markets in China during 2011 to 2013</title><author>Wang, Jingjing ; Liu, Hualei ; Liu, Wei ; Zheng, Dongxia ; Zhao, Yunling ; Li, Yin ; Wang, Yingli ; Ge, Shengqiang ; Lv, Yan ; Zuo, Yuanyuan ; Yu, Songmei ; Wang, Zhiliang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-50b509e7f857c97cb580dbc890677d846e1d549d392e79ffa216839b4eebd1793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animal vaccines</topic><topic>Animals</topic><topic>Antigenic determinants</topic><topic>Antigens</topic><topic>Birds</topic><topic>Birds - virology</topic><topic>Chemical properties</topic><topic>Cladistic analysis</topic><topic>Diagnostic software</topic><topic>Diagnostic systems</topic><topic>Epitopes</topic><topic>Fusion protein</topic><topic>Gene order</topic><topic>Gene sequencing</topic><topic>Genome, Viral - genetics</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Genotypes</topic><topic>Hemagglutination inhibition</topic><topic>Markets</topic><topic>Mutation</topic><topic>Newcastle disease</topic><topic>Newcastle disease virus - classification</topic><topic>Newcastle disease virus - genetics</topic><topic>Nucleotides</topic><topic>Outbreaks</topic><topic>Phylogeny</topic><topic>Pigeons</topic><topic>Poultry</topic><topic>Proteins</topic><topic>Transmembrane domains</topic><topic>Vaccines</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jingjing</creatorcontrib><creatorcontrib>Liu, Hualei</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Zheng, Dongxia</creatorcontrib><creatorcontrib>Zhao, Yunling</creatorcontrib><creatorcontrib>Li, Yin</creatorcontrib><creatorcontrib>Wang, Yingli</creatorcontrib><creatorcontrib>Ge, Shengqiang</creatorcontrib><creatorcontrib>Lv, Yan</creatorcontrib><creatorcontrib>Zuo, Yuanyuan</creatorcontrib><creatorcontrib>Yu, Songmei</creatorcontrib><creatorcontrib>Wang, Zhiliang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints In Context</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database (ProQuest)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Open Access: DOAJ - Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jingjing</au><au>Liu, Hualei</au><au>Liu, Wei</au><au>Zheng, Dongxia</au><au>Zhao, Yunling</au><au>Li, Yin</au><au>Wang, Yingli</au><au>Ge, Shengqiang</au><au>Lv, Yan</au><au>Zuo, Yuanyuan</au><au>Yu, Songmei</au><au>Wang, Zhiliang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic Characterizations of Six Pigeon Paramyxovirus Type 1 Viruses Isolated from Live Bird Markets in China during 2011 to 2013</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-04-30</date><risdate>2015</risdate><volume>10</volume><issue>4</issue><spage>e0124261</spage><epage>e0124261</epage><pages>e0124261-e0124261</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Conceived and designed the experiments: JW HL. Performed the experiments: JW DZ Y. Zhao Y. Li YW SG Y. Lv Y. Zuo SY. Analyzed the data: JW. Contributed reagents/materials/analysis tools: WL ZW. Wrote the paper: JW HL.</notes><notes>Competing Interests: The authors have declared that no competing interests exist.</notes><abstract>The genomes of six pigeon paramyxovirus type 1 (PPMV-1) isolated from symptomless pigeons in live poultry markets during the national active surveillance from 2011 to 2013 were sequenced and analyzed in this study. The complete genome lengths of all isolates were 15,192 nucleotides with the gene order of 3'-NP-P-M-F-HN-L-5'. All isolates had the same motif of 112RRQKRF117 at the cleavage site of the fusion protein, which was typical of velogenic Newcastle disease virus (NDV). Several mutations were identified in the functional domains of F and HN proteins, including fusion peptide, heptad repeat region, transmembrane domains and neutralizing epitopes. Phylogenetic analysis based on sequences of complete genomes and six genes revealed that all isolates belonged to genotype VI in class II, but at least 2 sub-genotypes were identified. Most isolates were placed into sub-genotype VIb with the exception of pi/GX/1015/13, which was classified in sub-genotype VIa. The obvious antigenic difference between PPMV-1 isolates and La Sota strain was found based on the R-value calculated by cross hemagglutination inhibition (HI) assay. These results provided the evidence that PPMV-1 could be detected from healthy pigeons, and our study may be useful in designing vaccines used in pigeon, and developing molecular diagnostic tools to monitor and prevent future PPMV-1 outbreaks.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25928057</pmid><doi>10.1371/journal.pone.0124261</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-04, Vol.10 (4), p.e0124261-e0124261 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1677376606 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Animal vaccines Animals Antigenic determinants Antigens Birds Birds - virology Chemical properties Cladistic analysis Diagnostic software Diagnostic systems Epitopes Fusion protein Gene order Gene sequencing Genome, Viral - genetics Genomes Genomics Genotypes Hemagglutination inhibition Markets Mutation Newcastle disease Newcastle disease virus - classification Newcastle disease virus - genetics Nucleotides Outbreaks Phylogeny Pigeons Poultry Proteins Transmembrane domains Vaccines Viruses |
| title | Genomic Characterizations of Six Pigeon Paramyxovirus Type 1 Viruses Isolated from Live Bird Markets in China during 2011 to 2013 |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T11%3A45%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genomic%20Characterizations%20of%20Six%20Pigeon%20Paramyxovirus%20Type%201%20Viruses%20Isolated%20from%20Live%20Bird%20Markets%20in%20China%20during%202011%20to%202013&rft.jtitle=PloS%20one&rft.au=Wang,%20Jingjing&rft.date=2015-04-30&rft.volume=10&rft.issue=4&rft.spage=e0124261&rft.epage=e0124261&rft.pages=e0124261-e0124261&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0124261&rft_dat=%3Cgale_plos_%3EA419035986%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-50b509e7f857c97cb580dbc890677d846e1d549d392e79ffa216839b4eebd1793%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1677376606&rft_id=info:pmid/25928057&rft_galeid=A419035986&rfr_iscdi=true |