Genomic Characterizations of Six Pigeon Paramyxovirus Type 1 Viruses Isolated from Live Bird Markets in China during 2011 to 2013

The genomes of six pigeon paramyxovirus type 1 (PPMV-1) isolated from symptomless pigeons in live poultry markets during the national active surveillance from 2011 to 2013 were sequenced and analyzed in this study. The complete genome lengths of all isolates were 15,192 nucleotides with the gene ord...

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Bibliographic Details
Published in:PloS one 2015-04, Vol.10 (4), p.e0124261-e0124261
Main Authors: Wang, Jingjing, Liu, Hualei, Liu, Wei, Zheng, Dongxia, Zhao, Yunling, Li, Yin, Wang, Yingli, Ge, Shengqiang, Lv, Yan, Zuo, Yuanyuan, Yu, Songmei, Wang, Zhiliang
Format: Article
Language:English
Subjects:
Animal vaccines
Animals
Antigenic determinants
Antigens
Birds
Birds - virology
Chemical properties
Cladistic analysis
Diagnostic software
Diagnostic systems
Epitopes
Fusion protein
Gene order
Gene sequencing
Genome, Viral - genetics
Genomes
Genomics
Genotypes
Hemagglutination inhibition
Markets
Mutation
Newcastle disease
Newcastle disease virus - classification
Newcastle disease virus - genetics
Nucleotides
Outbreaks
Phylogeny
Pigeons
Poultry
Proteins
Transmembrane domains
Vaccines
Viruses
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Summary:The genomes of six pigeon paramyxovirus type 1 (PPMV-1) isolated from symptomless pigeons in live poultry markets during the national active surveillance from 2011 to 2013 were sequenced and analyzed in this study. The complete genome lengths of all isolates were 15,192 nucleotides with the gene order of 3'-NP-P-M-F-HN-L-5'. All isolates had the same motif of 112RRQKRF117 at the cleavage site of the fusion protein, which was typical of velogenic Newcastle disease virus (NDV). Several mutations were identified in the functional domains of F and HN proteins, including fusion peptide, heptad repeat region, transmembrane domains and neutralizing epitopes. Phylogenetic analysis based on sequences of complete genomes and six genes revealed that all isolates belonged to genotype VI in class II, but at least 2 sub-genotypes were identified. Most isolates were placed into sub-genotype VIb with the exception of pi/GX/1015/13, which was classified in sub-genotype VIa. The obvious antigenic difference between PPMV-1 isolates and La Sota strain was found based on the R-value calculated by cross hemagglutination inhibition (HI) assay. These results provided the evidence that PPMV-1 could be detected from healthy pigeons, and our study may be useful in designing vaccines used in pigeon, and developing molecular diagnostic tools to monitor and prevent future PPMV-1 outbreaks.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0124261