Dopamine modulates insulin release and is involved in the survival of rat pancreatic beta cells

The local synthesis of dopamine and its effects on insulin release have been described in isolated islets. Thus, it may be accepted that dopamine exerts an auto-paracrine regulation of insulin secretion from pancreatic beta cells. The aim of the present study is to analyze whether dopamine is a regu...

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Bibliographic Details
Published in:PloS one 2015-04, Vol.10 (4), p.e0123197-e0123197
Main Authors: Garcia Barrado, Maria Jose, Iglesias Osma, Maria Carmen, Blanco, Enrique J, Carretero Hernández, Marta, Sánchez Robledo, Virginia, Catalano Iniesta, Leonardo, Carrero, Sixto, Carretero, Jose
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - drug effects
Beta cells
Caspase
Caspase-3
Cell proliferation
Cell Proliferation - drug effects
Cell survival
Cell Survival - drug effects
Cytochemistry
Dopamine
Dopamine - pharmacology
Glucose
Glucose - pharmacology
Insulin
Insulin - metabolism
Insulin Secretion
Insulin-Secreting Cells - drug effects
Insulin-Secreting Cells - metabolism
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Labeling
Labelling
Male
Pancreas
Pancreatic beta cells
Paracrine signalling
Phenols (Class of compounds)
Proliferating cell nuclear antigen
Rats
Rats, Wistar
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Summary:The local synthesis of dopamine and its effects on insulin release have been described in isolated islets. Thus, it may be accepted that dopamine exerts an auto-paracrine regulation of insulin secretion from pancreatic beta cells. The aim of the present study is to analyze whether dopamine is a regulator of the proliferation and apoptosis of rat pancreatic beta cells after glucose-stimulated insulin secretion. Glucose stimulated pancreatic islets obtained from male Wistar rats were cultured with 1 or 10 μM dopamine from 1 to 12 h. Insulin secretion was analyzed by RIA. The cellular proliferation rate of pancreatic islets and beta cells was studied with immunocytochemical double labelling for both insulin and PCNA (proliferating cell nuclear antigen), and active caspase-3 was detected to evaluate apoptosis. The secretion of insulin from isolated islets was significantly inhibited (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0123197