Physiological and comparative proteomic analysis reveals different drought responses in roots and leaves of drought-tolerant wild wheat (Triticum boeoticum)

To determine the proteomic-level responses of drought tolerant wild wheat (Triticum boeoticum), physiological and comparative proteomic analyses were conducted using the roots and the leaves of control and short term drought-stressed plants. Drought stress was imposed by transferring hydroponically...

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Bibliographic Details
Published in:PloS one 2015-04, Vol.10 (4), p.e0121852-e0121852
Main Authors: Liu, Hui, Sultan, Muhammad Abdul Rab Faisal, Liu, Xiang Li, Zhang, Jin, Yu, Fei, Zhao, Hui Xian
Format: Article
Language:English
Subjects:
Abscisic acid
Abscisic Acid - metabolism
Acids
Amino acids
Analysis
Biology
Carbon
Carbon fixation
Chaperones
Climate change
Complexity
Detoxification
Drought
Drought resistance
Droughts
Electrophoresis, Gel, Two-Dimensional
Energy metabolism
Environmental aspects
Environmental conditions
Glucose metabolism
Glycine max
Glycolysis
Homeostasis
Hydroponics
Influence
Laboratories
Leaves
Metabolism
Nitrogen metabolism
Organs
Oryza
Photosynthesis
Physiological aspects
Physiology
Plant Leaves - metabolism
Plant Proteins - metabolism
Plant Roots - metabolism
Plants
Protein metabolism
Protein turnover
Proteins
Proteome - analysis
Proteomics
Rice
Roots
Seedlings
Seedlings - metabolism
Short term
Soybeans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Studies
Transcription
Transduction
Triticum
Triticum - growth & development
Triticum - metabolism
Triticum aestivum
Triticum boeoticum
Wheat
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Summary:To determine the proteomic-level responses of drought tolerant wild wheat (Triticum boeoticum), physiological and comparative proteomic analyses were conducted using the roots and the leaves of control and short term drought-stressed plants. Drought stress was imposed by transferring hydroponically grown seedlings at the 3-leaf stage into 1/2 Hoagland solution containing 20% PEG-6000 for 48 h. Root and leaf samples were separately collected at 0 (control), 24, and 48 h of drought treatment for analysis. Physiological analysis indicated that abscisic acid (ABA) level was greatly increased in the drought-treated plants, but the increase was greater and more rapid in the leaves than in the roots. The net photosynthetic rate of the wild wheat leaves was significantly decreased under short-term drought stress. The deleterious effects of drought on the studied traits mainly targeted photosynthesis. Comparative proteomic analysis identified 98 and 85 differently changed protein spots (DEPs) (corresponding to 87 and 80 unique proteins, respectively) in the leaves and the roots, respectively, with only 6 mutual unique proteins in the both organs. An impressive 86% of the DEPs were implicated in detoxification and defense, carbon metabolism, amino acid and nitrogen metabolism, proteins metabolism, chaperones, transcription and translation, photosynthesis, nucleotide metabolism, and signal transduction. Further analysis revealed some mutual and tissue-specific responses to short-term drought in the leaves and the roots. The differences of drought-response between the roots and the leaves mainly included that signal sensing and transduction-associated proteins were greatly up-regulated in the roots. Photosynthesis and carbon fixation ability were decreased in the leaves. Glycolysis was down-regulated but PPP pathway enhanced in the roots, resulting in occurrence of complex changes in energy metabolism and establishment of a new homeostasis. Protein metabolism was down-regulated in the roots, but enhanced in the leaves. These results will contribute to the existing knowledge on the complexity of root and leaf protein changes that occur in response to drought, and also provide a framework for further functional studies on the identified proteins.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0121852