Multi-lineage differentiation of human umbilical cord Wharton's Jelly Mesenchymal Stromal Cells mediates changes in the expression profile of stemness markers

Wharton's Jelly- derived Mesenchymal stem cells (WJ-MSCs) have gained interest as an alternative source of stem cells for regenerative medicine because of their potential for self-renewal, differentiation and unique immunomodulatory properties. Although many studies have characterized various W...

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Bibliographic Details
Published in:PloS one 2015-04, Vol.10 (4), p.e0122465-e0122465
Main Authors: Ali, Hamad, Al-Yatama, Majda K, Abu-Farha, Mohamed, Behbehani, Kazem, Al Madhoun, Ashraf
Format: Article
Language:English
Subjects:
Adipogenesis
Adult
Analysis
Antigens
Antigens, Differentiation - biosynthesis
Biocompatibility
Biomarkers
Biomedical materials
Bone marrow
CD105 antigen
CD29 antigen
CD44 antigen
CD73 antigen
CD90 antigen
Cell adhesion & migration
Cell Differentiation
Cell self-renewal
Cells, Cultured
Chondrogenesis
Diabetes
Differentiation
Ethics
Female
Flow cytometry
Gene expression
Gene Expression Regulation
Health sciences
Humans
Immunofluorescence
Immunomodulation
Infant, Newborn
Laboratories
Male
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Mesenchyme
Molecular biology
Osteogenesis
Regenerative medicine
Signal transduction
Stem cells
Stromal cells
Surface markers
Umbilical cord
Umbilical Cord - cytology
Umbilical Cord - metabolism
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Summary:Wharton's Jelly- derived Mesenchymal stem cells (WJ-MSCs) have gained interest as an alternative source of stem cells for regenerative medicine because of their potential for self-renewal, differentiation and unique immunomodulatory properties. Although many studies have characterized various WJ-MSCs biologically, the expression profiles of the commonly used stemness markers have not yet been addressed. In this study, WJ-MSCs were isolated and characterized for stemness and surface markers expression. Flow cytometry, immunofluorescence and qRT-PCR analysis revealed predominant expression of CD29, CD44, CD73, CD90, CD105 and CD166 in WJ-MSCs, while the hematopoietic and endothelial markers were absent. Differential expression of CD 29, CD90, CD105 and CD166 following adipogenic, osteogenic and chondrogenic induction was observed. Furthermore, our results demonstrated a reduction in CD44 and CD73 expressions in response to the tri-lineage differentiation induction, suggesting that they can be used as reliable stemness markers, since their expression was associated with undifferentiated WJ-MSCs only.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0122465