Metagenomic profile of the viral communities in Rhipicephalus spp. ticks from Yunnan, China

Besides mosquitoes, ticks are regarded as the primary source of vector-borne infectious diseases. Indeed, a wide variety of severe infectious human diseases, including those involving viruses, are transmitted by ticks in many parts of the world. To date, there are no published reports on the use of...

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Bibliographic Details
Published in:	PloS one 2015-03, Vol.10 (3), p.e0121609-e0121609
Main Authors:	Xia, Han, Hu, Cheng, Zhang, Dake, Tang, Shuang, Zhang, Zhong, Kou, Zheng, Fan, Zhaojun, Bente, Dennis, Zeng, Changqing, Li, Tianxian
Format:	Article
Language:	eng
Subjects:	Amblyomma americanum Anelloviridae - genetics Anelloviridae - isolation & purification Animals Aquatic insects Arachnid Vectors - virology Arachnids Arthropods Bacteriophages - genetics Bacteriophages - isolation & purification Boophilus Bunyaviridae Cattle China Culicidae Disease transmission Fever Genome, Viral Genomes Genomics Haemaphysalis High-Throughput Nucleotide Sequencing Immunology Infections Infectious diseases Laboratories Livestock Metagenomics Mosquitoes Nairovirus - genetics Nairovirus - isolation & purification Parasitic diseases Phylogeny Plant Viruses - genetics Plant Viruses - isolation & purification Rhabdoviridae - genetics Rhabdoviridae - isolation & purification Rhipicephalus Rhipicephalus - classification Rhipicephalus - genetics Rhipicephalus - virology Ribosomal DNA Sheep Ticks Vector-borne diseases Virology Viruses
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Summary:	Besides mosquitoes, ticks are regarded as the primary source of vector-borne infectious diseases. Indeed, a wide variety of severe infectious human diseases, including those involving viruses, are transmitted by ticks in many parts of the world. To date, there are no published reports on the use of next-generation sequencing for studying viral diversity in ticks or discovering new viruses in these arthropods from China. Here, Ion-torrent sequencing was used to investigate the presence of viruses in three Rhipicephalus spp. tick pools (NY-11, NY-13, and MM-13) collected from the Menglian district of Yunnan, China. The sequencing run resulted in 3,641,088, 3,106,733, and 3,871,851 reads in each tick pool after trimming. Reads and assembled contiguous sequences (contigs) were subject to basic local alignment search tool analysis against the GenBank database. Large numbers of reads and contigs related to known viral sequences corresponding to a broad range of viral families were identified. Some of the sequences originated from viruses that have not been described previously in ticks. Our findings will facilitate better understanding of the tick virome, and add to our current knowledge of disease-causing viruses in ticks living under natural conditions.
ISSN:	1932-6203 1932-6203